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Forum: Sample Prep / Library Generation 05-29-2020, 07:44 PM
Replies: 5
Views: 2,691
Posted By ychang
https://sagescience.com/wp-content/uploads/2020/03...

https://sagescience.com/wp-content/uploads/2020/03/AGBT-202-POSTER-BO-ZHOU.pdf
I think this is the best method for your purpose.
Forum: Literature Watch 05-29-2020, 06:43 PM
Replies: 0
Views: 1,625
Posted By ychang
very simplified whole genome sequencing library preparation process

https://plantmethods.biomedcentral.com/articles/10.1186/s13007-020-00615-3

suitable for genetic population or small size genome.
Forum: Sample Prep / Library Generation 05-29-2020, 06:34 PM
Replies: 1
Views: 1,361
Posted By ychang
if you want whole genome resequencing, I can...

if you want whole genome resequencing, I can help!
https://plantmethods.biomedcentral.com/articles/10.1186/s13007-020-00615-3
Forum: Sample Prep / Library Generation 07-04-2019, 02:15 AM
Replies: 6
Views: 2,833
Posted By ychang
Hi,microgirl, your idea of using a qPCR...

Hi,microgirl,

your idea of using a qPCR instrument as a Qubit reader was great! could you give more detail of the protocol? Thanks!
Forum: Sample Prep / Library Generation 05-19-2014, 01:48 PM
Replies: 0
Views: 2,473
Posted By ychang
efficiency of end repair, A-tailing and adaptor ligation

Does anyone have the idea of how efficiency it was during NGS library prep? I am wondering at each step, the efficiency will lower than 50%, but have no idea to check it?
Forum: Illumina/Solexa 12-13-2013, 06:33 AM
Replies: 44
Views: 20,387
Posted By ychang
when we get the illumina training one year...

when we get the illumina training one year before, the FAS have told us to use 0.1N NaOH. it is interesting that yaximik use 1M acetic acid before loading on FC, have anyone tried HCl?
Forum: Illumina/Solexa 08-09-2013, 11:59 AM
Replies: 7
Views: 4,302
Posted By ychang
it is not a perfect library because the fragment...

it is not a perfect library because the fragment is too large, the ratio of DNA/transponase is very sensitive to the insert fragment, I will use a little more enzyme when I do next time. however, if...
Forum: Illumina/Solexa 08-07-2013, 08:50 AM
Replies: 1
Views: 1,923
Posted By ychang
we have solved the problem with the help of...

we have solved the problem with the help of Illumina and Genologics, now it works good.
Forum: Illumina/Solexa 07-30-2013, 09:54 AM
Replies: 31
Views: 16,417
Posted By ychang
Does anyone try the method described in the PNAS...

Does anyone try the method described in the PNAS paper?

http://www.pnas.org/content/110/14/5552.abstract

I am trying it now and want to share the experience with our colleagues!
Forum: Illumina/Solexa 04-05-2013, 01:14 PM
Replies: 1
Views: 1,923
Posted By ychang
Hiseq 2500 and LIMS is not compatible well!

Just a reminder that maybe the Hiseq 2500 (not upgrade from 2000) is not compatible with LIMS well.

We installed a Hiseq 2500 in Feb, it runs good! but after we installed the LIMS, the hiseq...
Forum: Sample Prep / Library Generation 03-05-2013, 03:39 PM
Replies: 5
Views: 5,164
Posted By ychang
Smile For Nextera mate-pair kit, Is it possible to reduce the tagmentation reaction time?

For Nextera mate-pair kit, Is it possible to reduce the tagmentation reaction time to get more long fragment, say 10kb? we have use this kit and found most of the smear is around2-7kb, but we want to...
Forum: Sample Prep / Library Generation 03-05-2013, 03:09 PM
Replies: 10
Views: 4,282
Posted By ychang
we have succeed make 4-7kb mate-pair lib with...

we have succeed make 4-7kb mate-pair lib with Nextera kit, and will try 10kb this week.
Forum: Sample Prep / Library Generation 02-05-2013, 04:55 PM
Replies: 1
Views: 1,101
Posted By ychang
can not get 1ug library to enrich

I am using trueseq kit to make library for enrichment, but I can always get only 400ng library, even I increase the input from 1ug to 5ug, the other thing is that whatever how much DNA was inputed,...
Forum: Illumina/Solexa 02-03-2013, 04:41 PM
Replies: 1
Views: 2,373
Posted By ychang
to my knowledge, if A and B were added to the...

to my knowledge, if A and B were added to the same fragment, it will not be amplified or amplified with low efficiency due to the formation of pot shape (suppression PCR).
Forum: Sample Prep / Library Generation 04-28-2011, 12:08 PM
Replies: 4
Views: 4,949
Posted By ychang
What is the longest fragment for Illumina Mate pair end seq library?

Hi, every, I want to prepare a library for mate pair end seq, the special aspect of the library is that I want the length of the fragment as long as possible, say, 10kb or even 20kb. Do you think the...
Forum: Sample Prep / Library Generation 10-12-2010, 05:01 PM
Replies: 3
Views: 2,030
Posted By ychang
How about we chat by QQ? my Email:...

How about we chat by QQ? my Email: imershay@gmail.com
Forum: Sample Prep / Library Generation 10-12-2010, 07:11 AM
Replies: 3
Views: 2,030
Posted By ychang
you want to sequence the repeat seqs or remove...

you want to sequence the repeat seqs or remove the repeat seq from the library?
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