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Forum: Sample Prep / Library Generation 03-02-2020, 08:38 AM
Replies: 1
Views: 1,217
Posted By ES5063
Admins if you want to move/delete this thread...

Admins if you want to move/delete this thread please feel, it may have been better placed under Sample Prep.

As information for anyone else, Fisher and SeqMatic also sell these tubes (produced by...
Forum: Sample Prep / Library Generation 02-17-2020, 08:51 AM
Replies: 1
Views: 1,217
Posted By ES5063
Gel breaker tube alternatives

We routinely use gel breaker tubes from IST Engineering after cutting our libraries out of gels, as recommended by our Illumina library prep. I've just been told there are some issues with IST and...
Forum: Illumina/Solexa 10-24-2019, 02:10 AM
Replies: 6
Views: 1,322
Posted By ES5063
Honestly using the 300 cycles was a learning...

Honestly using the 300 cycles was a learning curve. Previously only needed 50 cycles kits so was initially told you put down the number of cycles of the kit in the sample sheet. The idea of...
Forum: Illumina/Solexa 10-23-2019, 05:10 AM
Replies: 6
Views: 1,322
Posted By ES5063
Cluster density was higher than I anticipated,...

Cluster density was higher than I anticipated, loaded 6 pM and density was 1300K/mm2. I'll keep that in mind to load less than I typically would for future runs.

Yup, hoping to have tech support...
Forum: Illumina/Solexa 10-23-2019, 04:59 AM
Replies: 6
Views: 1,322
Posted By ES5063
The span that I cut when size selecting on a gel....

The span that I cut when size selecting on a gel. Prior libraries have been ~140-160 bp (total library size). The new ones are more like ~140-340 bp.
Forum: Illumina/Solexa 10-23-2019, 04:46 AM
Replies: 6
Views: 1,322
Posted By ES5063
MiSeq tolerated library size distribution

Our lab is trying some new library generation protocols where we are size selecting a much broader range of libraries than we have run previously, a 200 bp span as opposed to a 20 bp span. When we...
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