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Forum: Literature Watch 01-11-2013, 01:49 PM
Replies: 19
Views: 16,314
Posted By ashes11
It seems that the DTT in NEB Buffer 4...

It seems that the DTT in NEB Buffer 4 precipitates COCl2 in the reaction solution. T-tailing of an internal control (50 ng PCR fragment, 360 bp) does not work in my hands when using NEB Buffer 4, 5mM...
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