SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 12 of 12
Search took 0.00 seconds.
Search: Posts Made By: isalarubi
Forum: Ion Torrent 04-22-2018, 02:46 AM
Replies: 5
Views: 4,195
Posted By isalarubi
Dear @sentmcom, could you put me in contact...

Dear @sentmcom,
could you put me in contact with the this group using the Ion torrent pipeline? I have tried to use it, but although the accuracy of the prediction is really good, it has a lot of...
Forum: 454 Pyrosequencing 12-03-2013, 05:53 AM
Replies: 6
Views: 2,541
Posted By isalarubi
Sorry!:( The sff file only has 2kb. This run...

Sorry!:(

The sff file only has 2kb. This run was a totally disaster, so I think that we can forget it.

Thanks so much, and sorry, I'm totally new at this and I don't understand the most of the...
Forum: 454 Pyrosequencing 12-02-2013, 06:36 AM
Replies: 6
Views: 2,541
Posted By isalarubi
Hi Glovesfox! Finally, I couldn't generate...

Hi Glovesfox!

Finally, I couldn't generate the files, so if you have the same problem, I can't say you anything.

Sorry!

Isabel
Forum: 454 Pyrosequencing 11-21-2013, 07:34 AM
Replies: 11
Views: 3,226
Posted By isalarubi
Thanks a lot NEwmoon, we are in touch with...

Thanks a lot NEwmoon,

we are in touch with the technical support from Roche, and well, they have said that the machine is ok, and that it could be only an isolated event, and I hope so.

We are...
Forum: 454 Pyrosequencing 11-18-2013, 02:44 AM
Replies: 11
Views: 3,226
Posted By isalarubi
Dear newmoon, sorry, for the wrong file, I...

Dear newmoon,

sorry, for the wrong file, I think this new one is the good one.

Thank you!!

Isabel
Forum: 454 Pyrosequencing 11-12-2013, 05:02 AM
Replies: 3
Views: 1,941
Posted By isalarubi
Dear Newmoon, we didn't mix experiments, and...

Dear Newmoon,
we didn't mix experiments, and when we sent the report to the technical service, they only told us that it could be a warning with the enzime chiller temperature plot, but we don't...
Forum: 454 Pyrosequencing 11-12-2013, 04:51 AM
Replies: 11
Views: 3,226
Posted By isalarubi
Dear Newmoon, here you have the files. I...

Dear Newmoon,

here you have the files. I hope you can give us some information from them.

Thank you!
Forum: 454 Pyrosequencing 11-11-2013, 05:35 AM
Replies: 3
Views: 1,941
Posted By isalarubi
No key wells

Hi everyone!

could anybody explain me what a "no key" in the GS Run Browser application’s Wells tab means?, because we are having a lot of these wells.

thank you

Isabel
Forum: 454 Pyrosequencing 11-04-2013, 01:38 PM
Replies: 6
Views: 2,541
Posted By isalarubi
sff into fasta/qual files

Hi everybody!

I have a problem: when I have seen my fasta/qual files of my 454Jn run, I've realized that these files are 0kb, totally empty!!:eek:

anybody knows what happens?

thank you!!
...
Forum: 454 Pyrosequencing 11-04-2013, 06:44 AM
Replies: 11
Views: 3,226
Posted By isalarubi
Thank you Mike, we are using Lib L....

Thank you Mike,

we are using Lib L. Definitely we need to be more conservative with the bead:template ratio, so that we were working with a higher one as in the training told us, but in our case...
Forum: 454 Pyrosequencing 11-04-2013, 05:17 AM
Replies: 11
Views: 3,226
Posted By isalarubi
Thank you ajthomas, but we are quite sure that we...

Thank you ajthomas, but we are quite sure that we did not use the primers in the wrong way.

We do not understand the program very well yet. Probably our answer would be in the files, but we do not...
Forum: 454 Pyrosequencing 10-31-2013, 06:12 AM
Replies: 11
Views: 3,226
Posted By isalarubi
Unhappy Problems with 16S sequencing 454Jr

Hi everybody!

We're new with the 454 Jr, and in our first run of 16S to determine the stool microbiota we found some problems.

We've had rawWells,304998 and then only keyPassWells,12489. When...
Showing results 1 to 12 of 12

 


All times are GMT -8. The time now is 09:35 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO