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Forum: General 12-19-2014, 04:37 AM
Replies: 1
Views: 2,575
Posted By KJoseph
Currently, we put the betaine under heat until it...

Currently, we put the betaine under heat until it goes into solution with stirring.
Forum: General 12-19-2014, 04:11 AM
Replies: 1
Views: 2,575
Posted By KJoseph
Unhappy Proper way to prepare 5M Betaine monohydrate without oxidation?

Hello,

One of our buffers uses a stock of 5M betaine in which my lab prepares from dried betaine monohydrate. The buffer contains betaine, 10mm Tris-HCl, Tween 20, and water. We've tried DEPC...
Forum: Sample Prep / Library Generation 12-20-2013, 08:43 AM
Replies: 5
Views: 7,405
Posted By KJoseph
In my experience, that amount of adapter dimer...

In my experience, that amount of adapter dimer would cause some significant problems on a MiSeq, and you would definitely lose quite a number of reads on HiSeq as well.
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