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Forum: Sample Prep / Library Generation 02-26-2013, 12:25 PM
Replies: 0
Views: 2,783
Posted By MTTom
Optimal Insert Length for RNAseq on Illumina?

Hello,

I am planning on using RNAseq with Illumina to generate a de novo transcriptome for a non-model mammal species. I'll be using a 100bp PE protocol and multiplexing 24 samples in a lane. I...
Forum: Sample Prep / Library Generation 02-25-2013, 01:56 PM
Replies: 1
Views: 1,174
Posted By MTTom
I had an issue similar to this that I was able to...

I had an issue similar to this that I was able to fix it by diluting the sample. The bioanalyzer is highly sensitive to concentration and the Pico chip that you used is good between 50 and 5,000...
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