SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 11 of 11
Search took 0.00 seconds.
Search: Posts Made By: UCan'tBcereus
Forum: Illumina/Solexa 05-29-2019, 11:32 AM
Replies: 3
Views: 1,041
Posted By UCan'tBcereus
Hi BexMultiplex, According to the Nextera...

Hi BexMultiplex,

According to the Nextera Flex patent, TWB is:

-100 mM Tris HCl pH 8.5
-100 mM NaCL
-0.1% Tween 20
...
Forum: General 05-29-2019, 11:08 AM
Replies: 3
Views: 1,547
Posted By UCan'tBcereus
Hi Micro151086, it looks like you have a...

Hi Micro151086,

it looks like you have a lot of tRNA in your samples. tRNA is usually in that size range.
I have a few questions.

What Extraction method are you using? Some kits have...
Forum: RNA Sequencing 05-29-2019, 10:56 AM
Replies: 1
Views: 1,116
Posted By UCan'tBcereus
Hi seqtechno1, Issues could be: ...

Hi seqtechno1,

Issues could be:

-Ribodepletion Kit used does not target tRNAs. If they are targeted then they will not be removed. Most kits use a pretty high (2.0x or above) bead cleanup, so...
Forum: Sample Prep / Library Generation 04-30-2019, 09:22 AM
Replies: 4
Views: 1,062
Posted By UCan'tBcereus
Whoops! The thread I meant to show is: ...

Whoops!

The thread I meant to show is:

http://seqanswers.com/forums/showthread.php?t=71989

Besides the BSA (or other residual proteins), the only other thing that I have seen issues with...
Forum: Sample Prep / Library Generation 04-30-2019, 04:05 AM
Replies: 4
Views: 1,062
Posted By UCan'tBcereus
Hi teuthbrush, See the thread below that may...

Hi teuthbrush,

See the thread below that may interest you:

http://seqanswers.com/forums/showthread.php?t=89063

When you say you did dummy reactions with no enzymes, did you include the...
Forum: Illumina/Solexa 02-22-2019, 04:06 AM
Replies: 13
Views: 9,550
Posted By UCan'tBcereus
Hi kternus, We have also also worked on some...

Hi kternus,

We have also also worked on some very low input Netera XT samples as well, although we were working on metagenomic samples too.

We used the following paper from the Phillip...
Forum: Sample Prep / Library Generation 09-21-2018, 08:43 AM
Replies: 2
Views: 1,411
Posted By UCan'tBcereus
Nextera Flex 1/2 and 1/4 Reaction Experiences?

Hi Everyone,

We are about to run through a test of the Nextera Flex kit, and I wanted to see if anyone has had a positive experience cutting down the RXN volume down to 1/2 RXN or 1/4 RXN.

When...
Forum: Sample Prep / Library Generation 08-31-2018, 05:18 AM
Replies: 6
Views: 1,373
Posted By UCan'tBcereus
Hi Phillip, There was some confusion in our...

Hi Phillip,

There was some confusion in our lab about this, but it was only the Nextera (not the Nextera XT) kit that was discontinued.

They also discontinued the Nextera XT Index Kit. Now...
Forum: Illumina/Solexa 08-27-2018, 06:25 AM
Replies: 6
Views: 1,657
Posted By UCan'tBcereus
Hi Pete, Glad to be of help. Also, just to...

Hi Pete,

Glad to be of help. Also, just to be clear, when I say successful library prep, I mean that the Library looks good and sequenced/clustered well. As far as actual quality of data, I cannot...
Forum: Illumina/Solexa 08-27-2018, 04:27 AM
Replies: 6
Views: 1,657
Posted By UCan'tBcereus
Hi Pete, Never used the Truseq Kit before,...

Hi Pete,

Never used the Truseq Kit before, but our lab uses Scriptseq Complete Gold Epidemiology Kit + RiboZero when we make RNASeq Libraries. We use epidemiology kit since we are often working on...
Forum: Sample Prep / Library Generation 08-27-2018, 04:06 AM
Replies: 4
Views: 1,551
Posted By UCan'tBcereus
Hi Whe, We have a Tecan Freedom Evo 150 in...

Hi Whe,

We have a Tecan Freedom Evo 150 in our lab (8 channel head only). We for the most part like it. There is a learning curve to it, but if you take the time to learn the software there’s a...
Showing results 1 to 11 of 11

 


All times are GMT -8. The time now is 02:45 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO