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Search: Posts Made By: Marķa Camila Fetiva
Forum: Sample Prep / Library Generation 03-01-2019, 05:55 AM
Replies: 7
Views: 2,621
Does my library look ok?

Hello, I performed ATACseq preparation of librarys I used 50.000 cells counted by en bauer chamber, and following the protocol of Buen rostro, I did 25 cycles in total of PCR. Can someone tell me if...
Forum: Sample Prep / Library Generation 03-01-2019, 05:50 AM
Replies: 9
Views: 3,194
ATAC seq

Hello, I performed ATACseq preparation of librarys I used 50.000 cells counted by en bauer chamber, and following the protocol of Buen rostro, I did 25 cycles in total of PCR. Can someone tell me if...
Forum: Sample Prep / Library Generation 02-27-2019, 12:33 PM
Replies: 10
Views: 4,964
thanks for your reply, do you think this Lybrary...

thanks for your reply, do you think this Lybrary look ok? or there is not banding pattern, i am not sure if it is ok to sequence.
Forum: Sample Prep / Library Generation 02-27-2019, 04:49 AM
Replies: 10
Views: 4,964
Hello this is the result I obtained and...

Hello this is the result I obtained and corresponds to sample number 1 please ignore the others.
I am following Buenrostro protocol in 50.000 cells. I did first 5 PCR cycles and next I added 20 PCR...
Forum: Sample Prep / Library Generation 01-24-2019, 04:57 AM
Replies: 10
Views: 4,964
Hello, Nowadays I am also having similar...

Hello,

Nowadays I am also having similar problems in my ATAC-seq protocol i don't have the banding pattern, could you finally resolve it and perform the ATAC-seq?.

Can you give me some...
Forum: Illumina/Solexa 09-07-2017, 05:47 AM
Replies: 3
Views: 1,202
Hello we have pooled 3 samples, we loaded library...

Hello we have pooled 3 samples, we loaded library in 1.25nM (1 assay with overlustering CLusters Density: 1909 k/mm2, PF: 67%), after that we loaded library to 1.0nM (Clusters Density: 137 k/mm2, PF:...
Forum: Illumina/Solexa 08-31-2017, 09:04 AM
Replies: 3
Views: 1,202
Question Trusight One panel Illumina

Hello, I have been working with trusight One panel from illumina, and it has been so difficult to standarize, and we have had oversultering and underclustering issues. We are working with a Miseq. We...
Forum: Illumina/Solexa 08-04-2017, 12:27 PM
Replies: 1
Views: 1,390
Question Miseq- error

Hello,
My miseq showed an error and stoped running two hours after the run started.
Error: Through- focus peak did not exceed SNR threshold...
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