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Forum: Metagenomics 11-26-2019, 05:11 AM
Replies: 2
Views: 3,072
Posted By seanw
I too am having the issue when looking for...

I too am having the issue when looking for endophytes in leaf and stem tissue. Qiagen DNeasy as well as Qiagen Kingfisher soil kit extractions then using V4 region as well as V5/6 are not working...
Forum: Sample Prep / Library Generation 06-04-2018, 06:42 AM
Replies: 3
Views: 1,116
Posted By seanw
Follow up info.

Hi,

here is some clarification on things.

There is no stagger within the primer pairs to increase the diversity and I have not added anything else, but this had not been an issue before (I...
Forum: Sample Prep / Library Generation 05-31-2018, 06:40 AM
Replies: 3
Views: 1,116
Posted By seanw
16S Library Plant Tissue Troubles

Hi all I thought I would seek advice here as I have tried a number of tweaks and not getting the results I would expect. Here is the background

DNA was extracted from plant stems to allow for a...
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