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Forum: Sample Prep / Library Generation 04-21-2016, 06:04 AM
Replies: 1
Views: 1,194
Posted By Susanne
Clearly, I am biassed, but the 4200 TapeStation...

Clearly, I am biassed, but the 4200 TapeStation from Agilent would probably do the job - depending on your needs....
Forum: Sample Prep / Library Generation 06-10-2014, 12:38 AM
Replies: 6
Views: 3,370
Posted By Susanne
Could you share an electropherogram image of the...

Could you share an electropherogram image of the issue, please?
Forum: Sample Prep / Library Generation 06-21-2013, 05:57 AM
Replies: 2
Views: 6,090
Posted By Susanne
The HS chip is quite sensitive with regards to...

The HS chip is quite sensitive with regards to genomic DNA and overloading. So I would recommend not to load more than 50 ng/Ál, better around 10-15 ng/Ál if you want to quantify. There are several...
Forum: 454 Pyrosequencing 10-01-2012, 06:30 AM
Replies: 10
Views: 6,059
Posted By Susanne
How does it look like if you turn off the...

How does it look like if you turn off the analysis in the Bioanalyzer (stop button)? Maybe you could also share one of the initial gel images for comparison?
Forum: Sample Prep / Library Generation 08-28-2012, 01:32 AM
Replies: 49
Views: 73,527
Posted By Susanne
Re. size of Ampure beads, I've also been...

Re. size of Ampure beads, I've also been wondering and reading a bit, and by looking through the Hawkins 1998 patent, I stumbled upon Biomag particles (formerly from PerSeptive, that had been...
Forum: Sample Prep / Library Generation 05-07-2012, 06:18 AM
Replies: 6
Views: 6,757
Posted By Susanne
In case you are dealing with a smear, your...

In case you are dealing with a smear, your sensitivity of 0.5 ng/Ál for the DNA1000 doesn't hold true, as these are defined to be in a (sharp) peak.
For a smear, the sensitivity will be lower...
Forum: Sample Prep / Library Generation 03-09-2012, 12:32 AM
Replies: 13
Views: 15,117
Posted By Susanne
Unfortunately, in the comparison context, it...

Unfortunately, in the comparison context, it doesn't work to change the x-axis to size. Only time is available. :(
Forum: Illumina/Solexa 01-25-2012, 05:00 AM
Replies: 21
Views: 8,707
Posted By Susanne
We tested ssDNA (primer size, <100 nt) on the...

We tested ssDNA (primer size, <100 nt) on the Bioanalyzer DNA assays, and it does run slower than dsDNA of the same size (roughly -25-30%).
Also, the quantification for the ssDNA is off (may be...
Forum: Sample Prep / Library Generation 01-02-2012, 07:06 AM
Replies: 8
Views: 7,553
Posted By Susanne
...just right-click on the epg. image and save as...

...just right-click on the epg. image and save as :-)
Forum: Sample Prep / Library Generation 01-02-2012, 06:20 AM
Replies: 8
Views: 7,553
Posted By Susanne
Could you maybe share the electropherogram images...

Could you maybe share the electropherogram images from the chip? The gel images could be misleading sometimes, depending on their gray-scale. Thanks.
Forum: Sample Prep / Library Generation 10-31-2011, 05:44 AM
Replies: 10
Views: 11,108
Posted By Susanne
don't understand

Hi pmiguel, I don't exactly understand why you would run dsDNA on a RNA chip? It's not made for running DNA and prone to get the wrong results.
For what reason do you want to do that, what's the...
Forum: Sample Prep / Library Generation 02-07-2011, 08:07 AM
Replies: 49
Views: 73,527
Posted By Susanne
Does anybody know what the composition of the...

Does anybody know what the composition of the elution buffer is? Shouldn't that be almost water?
Forum: Sample Prep / Library Generation 08-27-2010, 05:06 AM
Replies: 13
Views: 15,117
Posted By Susanne
Referring to the time shown (x-axis) that changes...

Referring to the time shown (x-axis) that changes when the markers are changed (DNA chips): in order to calculate the correct sizes for all lanes, a 'virtual time' is assigned to the markers, and...
Forum: Sample Prep / Library Generation 08-27-2010, 04:57 AM
Replies: 13
Views: 15,117
Posted By Susanne
There's a little info inside the software on the...

There's a little info inside the software on the RIN - go to Help - Contents and Index. in the Application Notes under "Related Documents", there's something on RIN, and also you could search for RIN.
Forum: 454 Pyrosequencing 11-10-2009, 05:50 AM
Replies: 28
Views: 11,481
Posted By Susanne
Thanks for the info, Jason. Are you able to...

Thanks for the info, Jason.
Are you able to disclose what REM stands for?
No promotions yet on what that will be able to do?
Forum: General 11-06-2009, 07:52 AM
Replies: 8
Views: 7,945
Posted By Susanne
Thanks, Francesco for your quick and interesting...

Thanks, Francesco for your quick and interesting reply. I found the paper & will read it over the weekend.

I researched a bit and read that 454 was first to the market, as far as the 3 big players...
Forum: General 11-06-2009, 02:01 AM
Replies: 8
Views: 7,945
Posted By Susanne
Success of Solexa-platform

Hi members,
I am currently preparing an overview presentation for my science colleagues (unfamiliar with NGS) on the next- and now-gen sequencing technologies. Obviously, this forum is a great place...
Forum: General 11-05-2009, 07:46 AM
Replies: 9
Views: 24,901
Posted By Susanne
GATC webpage

I also think to have ssen more on comparisons somewhere on seqanswers, but also GATC offers info on their page:
http://www.gatc-biotech.com/en/sequencing.html
At least it mentions in part for what...
Forum: 454 Pyrosequencing 10-29-2009, 03:37 AM
Replies: 28
Views: 11,481
Posted By Susanne
Sorry - I don't have an answer to your question,...

Sorry - I don't have an answer to your question, but a qestion to you: where can I find the info (publicly) from Roche on the use of that REM e system? Can you post a link or PM me the info? Thanks...
Forum: Site Feedback/Suggestions 10-19-2009, 07:16 AM
Replies: 14
Views: 4,847
Posted By Susanne
Thanks a lot!! It seems like it's coming down...

Thanks a lot!!
It seems like it's coming down to personal preferences finally - one person would like to see the forums, somebody else may prefer having the post (snippet? - I think the feed...
Forum: Site Feedback/Suggestions 10-16-2009, 07:45 AM
Replies: 14
Views: 4,847
Posted By Susanne
report

Attensa didn't get 'all' new posts (Oct. 14+15+16) at once, however, after a second round of spitting them out, I received 50 posts in the feed (suspicious number, hmmm? :confused:).
It's probably...
Forum: Site Feedback/Suggestions 10-16-2009, 04:49 AM
Replies: 14
Views: 4,847
Posted By Susanne
Thumbs up Works!

Attensa was chewing on it a moment and then... there it was: all new posts! Cool :-)
THANKS!
Forum: Site Feedback/Suggestions 10-16-2009, 02:56 AM
Replies: 2
Views: 3,399
Posted By Susanne
New topic suggestion

How about a separate topic for target enrichment? It seems to be sneaking between the supplier's forums (Nimblegen) and sample prep and I feel it's being mentioned more lately.
I admit that it's a...
Forum: Site Feedback/Suggestions 10-16-2009, 02:45 AM
Replies: 14
Views: 4,847
Posted By Susanne
Any news on the "feed" story?

Hi ECO,
sure you're busy, also with the spam issue coming up (:mad: for what reason anybody spams this forum I will never understand :mad:)...
But I wanted to check back on the RSS-question asked...
Forum: Introductions 10-16-2009, 01:55 AM
Replies: 1
Views: 1,407
Posted By Susanne
Hi to Coumbia, MO from Germany - that's cool -...

Hi to Coumbia, MO from Germany - that's cool - I've been there for a while end really enjoyed it. Go Tigers! ;-)
So do you have a NGS sequencer in Columbia?
Showing results 1 to 25 of 33

 


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