SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 13 of 13
Search took 0.00 seconds.
Search: Posts Made By: MisUser
Forum: Illumina/Solexa 10-18-2017, 05:42 AM
Replies: 12
Views: 1,340
Posted By MisUser
Hi Provirus, Ask your local FSA if he/she...

Hi Provirus,

Ask your local FSA if he/she can provide you a custom recipe file with the spec you want. Illumina doesn't advertise it but they gave us the files necessary to run dark cycles (cycles...
Forum: Illumina/Solexa 03-29-2017, 10:59 AM
Replies: 9
Views: 1,350
Posted By MisUser
Thanks for your quick reply. The sequence is...

Thanks for your quick reply.
The sequence is identical for the first 25 nts and the diversity is good for the next 20nts.
Forum: Illumina/Solexa 03-29-2017, 07:01 AM
Replies: 9
Views: 1,350
Posted By MisUser
Low diversity on Nextseq 500

Hi all,

Does anyone have already sequenced low diversity libraries on the Nextseq and how did it perform? What were the conditions (PhiX % and library quantities)?
How many reads did you get? ...
Forum: Illumina/Solexa 03-29-2017, 06:56 AM
Replies: 1
Views: 786
Posted By MisUser
It was a hardware issue (Z-AMP PCB replaced)

It was a hardware issue (Z-AMP PCB replaced)
Forum: Illumina/Solexa 02-21-2017, 07:09 AM
Replies: 1
Views: 786
Posted By MisUser
Hiseq Initialization issue

Hi!

I'm about to start a Hiseq run but unfortunately the instrument cannot initialize even after several power cycles. We get this error message:

following device not initialized:...
Forum: Illumina/Solexa 02-08-2017, 10:33 AM
Replies: 8
Views: 1,317
Posted By MisUser
I got an answer from Illumina's tech support: ...

I got an answer from Illumina's tech support:
MiSeq v2 contains enough reagents for 75 cycles of chemistry
Forum: Illumina/Solexa 02-08-2017, 05:48 AM
Replies: 8
Views: 1,317
Posted By MisUser
Great news. Thank you for your answer Genomax!

Great news. Thank you for your answer Genomax!
Forum: Illumina/Solexa 02-08-2017, 05:28 AM
Replies: 8
Views: 1,317
Posted By MisUser
54 maximum + 6 for index

54 maximum + 6 for index
Forum: Illumina/Solexa 02-08-2017, 05:23 AM
Replies: 8
Views: 1,317
Posted By MisUser
Question Maximum number of cycles for kit Miseq 50 cycles

Hi sequencing Masters!

What is the maximum number of cycles we can do with a Miseq V2 50 cycles kit. I have been told that there is enough reagents for more than 55 cycles including the 6 cycles...
Forum: RNA Sequencing 01-26-2017, 10:30 AM
Replies: 3
Views: 1,855
Posted By MisUser
We experienced the same issue, until we diluted...

We experienced the same issue, until we diluted the RNAZap at 1:2 then the problem was solved.
Forum: Illumina/Solexa 01-23-2017, 12:47 PM
Replies: 8
Views: 1,956
Posted By MisUser
Hi nucacidhunter, Thank you for your input....

Hi nucacidhunter,

Thank you for your input. The client did not use a ligation process to prepare his libraries (instead he built them by PCR) but he used illumina's adapter sequences. We...
Forum: Illumina/Solexa 01-20-2017, 07:43 AM
Replies: 8
Views: 1,956
Posted By MisUser
Thank you for your quick reply, We had...

Thank you for your quick reply,

We had 1200k/mm2 but only 30% of them PF. After talking to the client, it may be possible that there is no A in the first 5 cycles of the read 2 but the library is...
Forum: Illumina/Solexa 01-20-2017, 06:53 AM
Replies: 8
Views: 1,956
Posted By MisUser
Miseq read 2 bad quality

Hi all,

We did a Miseq run (read 1-48 cycles, read 2-50 cycles) on a custom made library. The read quality for read 1 is OK but the read 2 quality is very low. Since it is an amplicon library, the...
Showing results 1 to 13 of 13

 


All times are GMT -8. The time now is 01:17 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO