Forum: Introductions
07-22-2010, 10:21 PM
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Replies: 373
Views: 179,410
Hi to all
hi,
I am a software engineer who was exposed to bioinformatics and sequencing two years back. Since then, i have been hooked on to this field. I have worked on Illumina short reads. Working on...
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Forum: Bioinformatics
06-28-2010, 01:16 AM
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Replies: 2
Views: 3,348
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Forum: Bioinformatics
06-24-2010, 06:10 AM
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Replies: 2
Views: 3,348
Samtools Merge and rmdup
hi,
I have 7 bam files which i need to merge into a single one.
1. Should i first sort the bam files and then merge or merge it first and then sort ?
2. Is it important to do a 'rmpup' ? Does...
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Forum: Bioinformatics
06-11-2010, 01:12 AM
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Replies: 2
Views: 5,103
Input to BWA from multiple Lanes
hi,
I have paired end data from multiple lanes (illumina pipleine) in qseq format.
i convert all the qseq files to fastq lane wise.
Combine the first sequences in one file and the next...
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Forum: Genomic Resequencing
06-08-2010, 03:11 AM
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Replies: 2
Views: 5,562
Thanks, for your immediate reply.
For (2.)...
Thanks, for your immediate reply.
For (2.) can we restrict the indels with the parameters -o and -e in the 'aln' step? What is the ideal value for these parameters given 115bp reads ?
4. i want...
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Forum: Genomic Resequencing
06-07-2010, 03:39 AM
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Replies: 2
Views: 5,562
BWA alignment for paired end reads
hi,
i am using BWA to align 115bp paired end solexa reads. I have the following doubts :-
1. Does BWA take the min and max insert sizes for paired end reads ?
2. What is maximum number of...
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Forum: Introductions
06-07-2010, 03:18 AM
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Replies: 0
Views: 1,062
Hi All
Hi all,
i am a new user and working on Solexa reads.
I am interested in working on various Bioinformatics Tools especially related to alignment.
Hope to gain a lot of help from all the...
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