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Forum: Sample Prep / Library Generation 03-04-2009, 07:24 AM
Replies: 5
Views: 16,946
Posted By EPICENTRE
There was another application by the FBI that...

There was another application by the FBI that looked at various ligation conditions prior to WGA.
http://www.promega.com/geneticidproc/ussymp19proc/oralpresentations/Nunez.pdf
Forum: Sample Prep / Library Generation 03-04-2009, 07:20 AM
Replies: 3
Views: 4,906
Posted By EPICENTRE
We are working on a way to fragment and add...

We are working on a way to fragment and add adaptors using a transposon-based method. Our fragments tend to smear in the 150-2000 bp range. How critical is it for you to be in the 150-200 bp range?...
Forum: Sample Prep / Library Generation 03-04-2009, 07:17 AM
Replies: 1
Views: 2,687
Posted By EPICENTRE
I don't think the 2-peptide strategy will work...

I don't think the 2-peptide strategy will work because each of the peptide will be independently displayed by the immune system. I know of a few companies that are trying this approach (with a single...
Forum: Sample Prep / Library Generation 03-04-2009, 07:07 AM
Replies: 2
Views: 4,799
Posted By EPICENTRE
My understanding is that they use a standard...

My understanding is that they use a standard A-tailing. The genomic fragments have 3'-A tails. The adaptors have a 5'-T overhang, not a 3'. This way you should get slightly improved ligation due to...
Forum: Introductions 03-03-2009, 05:38 AM
Replies: 0
Views: 923
Posted By EPICENTRE
Hello from beautiful Madison, WI...

I am working at a molecular biology company and am new to this forum and to NGS. We do not perform any NGS, but are working on some methods that may be helpful to folks in this area.

I look...
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