Forum: Introductions
11-11-2015, 02:12 PM
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Replies: 1
Views: 1,620
Hi Kurt,
Please get in touch directly....
Hi Kurt,
Please get in touch directly. james.hadfield@cruk.cam.ac.uk, @CIgenomics, or http://core-genomics.blogspot.co.uk.
I'd like to find out more and will take a look.
James.
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Forum: Sample Prep / Library Generation
11-11-2015, 02:06 PM
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Replies: 3
Views: 1,806
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Forum: Illumina/Solexa
11-11-2015, 01:52 PM
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Replies: 68
Views: 27,128
At Illumina's UK UGM the team discussed the low...
At Illumina's UK UGM the team discussed the low quality at the end of long-read MiSeq kits. They acknowleged there was an issue and that they were actively working on it. However they have no plans...
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Forum: Sample Prep / Library Generation
11-11-2015, 01:44 PM
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Replies: 2
Views: 1,935
Hi Alvaro and Martin,
We have seen drop out of...
Hi Alvaro and Martin,
We have seen drop out of the column 3 (D703) index on multiple RNA-seq runs. We are now very confident that this is not a simple problem. If we run the same library on multiple...
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Forum: Illumina/Solexa
08-15-2014, 05:56 AM
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Replies: 58
Views: 42,884
Hi, I just wrote a new post on my blog,...
Hi, I just wrote a new post on my blog, http://core-genomics.blogspot.co.uk/2014/08/how-many-miseq-owners-are-using-bleach.html which describes the bleach protocol and how to get it.
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Forum: Illumina/Solexa
08-15-2014, 05:54 AM
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Replies: 10
Views: 10,148
How to get the bleach protocol
Hi, I just wrote a new post on my blog, http://core-genomics.blogspot.co.uk/2014/08/how-many-miseq-owners-are-using-bleach.html which describes the bleach protocol and how to get it.
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Forum: Sample Prep / Library Generation
01-22-2014, 06:53 AM
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Replies: 20
Views: 10,823
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Forum: Sample Prep / Library Generation
01-22-2014, 06:51 AM
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Replies: 2
Views: 2,425
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Forum: Sample Prep / Library Generation
01-22-2014, 06:20 AM
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Replies: 4
Views: 2,665
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Forum: Sample Prep / Library Generation
12-16-2013, 11:08 AM
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Replies: 28
Views: 12,114
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Forum: Sample Prep / Library Generation
12-16-2013, 11:00 AM
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Replies: 20
Views: 10,823
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Forum: Illumina/Solexa
12-16-2013, 10:57 AM
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Replies: 12
Views: 6,686
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Forum: General
10-21-2013, 02:40 AM
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Replies: 15
Views: 10,089
Hmm, I'm not sure as I deal very much with the...
Hmm, I'm not sure as I deal very much with the wetlab side. I know we use Clarity as part of our automated analaysis but I believe we get the data out and use an in-house built workflow manager to...
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Forum: RNA Sequencing
10-21-2013, 02:35 AM
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Replies: 4
Views: 2,423
Option 3 every time.
You can multiplex up to...
Option 3 every time.
You can multiplex up to 96 samples in one pool on HiSeq. I always recommend pooling the whole experiment if possible, and if not consulting with a statistician beforehand to...
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Forum: Illumina/Solexa
10-21-2013, 02:33 AM
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Replies: 18
Views: 5,563
We found the bead normalisation was OK but...
We found the bead normalisation was OK but relying on it to achieve the correct cluster density was a wate of time. Most users want 1 good MiSeq run and the QT is too falky to guarantee this.
We...
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Forum: Illumina/Solexa
10-21-2013, 02:30 AM
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Replies: 2
Views: 2,286
ScottC's right. Good pipetting, large volumes...
ScottC's right. Good pipetting, large volumes (>5ul) and serial dilution till you get to the required concentration. Don't even try and use Nanodrop!
Hopefully Illumina et al will move to a bead...
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Forum: Illumina/Solexa
10-21-2013, 02:27 AM
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Replies: 2
Views: 2,992
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Forum: Illumina/Solexa
10-10-2013, 09:13 AM
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Replies: 4
Views: 2,775
Rapid exome
has anyone used the newest rapid exome capture kits from Ilumina. Our first run generated huge amounts of library pre-caprute and again in teh final PCR. I'm worried about over-amplifying and adding...
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Forum: RNA Sequencing
10-10-2013, 09:10 AM
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Replies: 9
Views: 10,715
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Forum: Epigenetics
10-10-2013, 09:08 AM
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Replies: 14
Views: 7,994
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Forum: Illumina/Solexa
10-10-2013, 09:04 AM
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Replies: 12
Views: 6,686
Dual-index barcode read failures
Hi All,
We've seen a lot of read 2 failures on our HiSeq and on externally supplied data. The quality of the second index is terrible and we lose most of the reads as they cannot be...
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Forum: General
10-10-2013, 08:53 AM
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Replies: 15
Views: 10,089
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Forum: Sample Prep / Library Generation
05-10-2013, 01:46 PM
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Replies: 16
Views: 31,510
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Forum: Sample Prep / Library Generation
05-09-2013, 11:33 PM
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Replies: 16
Views: 31,510
I'd seconddocbio's request for 384 barcodes ad...
I'd seconddocbio's request for 384 barcodes ad not just for Nextera XT. ChIP-seq and RNA-seq can often require more than 96 samples and we try to make a superpool and spread it across the number of...
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Forum: Illumina/Solexa
05-09-2013, 11:31 PM
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Replies: 7
Views: 3,180
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