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Forum: SOLiD 04-19-2012, 12:47 AM
Replies: 4
Views: 2,518
Posted By yksikaksi
Thanks, kopi-o. I'm doing RNA-seq analysis...

Thanks, kopi-o.

I'm doing RNA-seq analysis with SOLiD platform. I read people mentioned carry out quality filtering (someone also called it as pre-filtering) before mapping is recommended....
Forum: SOLiD 04-19-2012, 12:12 AM
Replies: 4
Views: 2,518
Posted By yksikaksi
Sorry for the unclear question. The...

Sorry for the unclear question.

The pre-filtering I refer here is view the reads with tools for example FastQC or Fastx and then trim out the so-called bad bases before mapping the reads.

The...
Forum: SOLiD 04-18-2012, 05:55 AM
Replies: 4
Views: 2,518
Posted By yksikaksi
Question What is the differences between pre-filtering vs PCR duplicates remove mapped reads?

Hello,

Does is a must to preform pre-filtering for color space reads before mapping?

Could it has a big differences in downstream analysis when I mapped the SOLiD color space reads without...
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