Forum: Illumina/Solexa
04-23-2020, 10:30 AM
|
|
Replies: 9
Views: 2,314
|
|
Forum: Illumina/Solexa
04-23-2020, 06:33 AM
|
|
Replies: 9
Views: 2,314
Thanks, ATϟGC, that's a good suggestion. ...
Thanks, ATϟGC, that's a good suggestion.
Looking back, the adapter-primers that I had used for my older runs when I didn't have this issue, did have some degenerate bases in between for different...
|
|
Forum: Illumina/Solexa
04-22-2020, 07:20 PM
|
|
Replies: 9
Views: 2,314
|
|
Forum: Illumina/Solexa
04-22-2020, 03:29 PM
|
|
Replies: 9
Views: 2,314
Thanks for your reply, GenoMax!
The sample is a...
Thanks for your reply, GenoMax!
The sample is a custom set of sequences with well-defined regions (hence those low-diversity regions). I had declined PhiX spike-in to obtain as many valid read lines...
|
|
Forum: Illumina/Solexa
04-22-2020, 12:04 PM
|
|
Replies: 9
Views: 2,314
Poor seq quality due to low diversity sample
Hi,
I have some sets of HiSeq data that I am analyzing and the sequencing quality turned out quite bad. I attach the "per base seq quality" diagram and the "per tile seq quality" diagram for one...
|
