Forum: Bioinformatics
04-10-2011, 07:51 PM
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Replies: 11
Views: 4,092
Luckily, dbSNP just released a fixed version in...
Luckily, dbSNP just released a fixed version in march (after I gone through all the hassle to get around the problem). They addressed the issue in the march release. I checked a few indel entries and...
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Forum: Bioinformatics
03-06-2011, 05:08 AM
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Replies: 24
Views: 7,276
the high through put sequencing technologies...
the high through put sequencing technologies might follow most other then-high-throughput technologies such as microarrays and that biology labs eventually would not have interest dealing with the...
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Forum: Bioinformatics
03-05-2011, 02:12 AM
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Replies: 24
Views: 7,276
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Forum: Bioinformatics
02-25-2011, 12:32 PM
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Replies: 0
Views: 2,017
dindel question
Hi all,
In the dindel manual there is a paragraph that reads:
Targeted resequencing: With targeted resequencing experiments, such as exome capture protocols, it is not possible to have reads on...
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Forum: Bioinformatics
02-25-2011, 06:12 AM
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Replies: 6
Views: 6,489
What did you mean by long rearrangements being...
What did you mean by long rearrangements being treated as short ones? I thought breakdancer outputs breakpoints info not about size of rearrangements. Could you clarify? I also agree with you, SV...
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Forum: Bioinformatics
02-18-2011, 11:55 AM
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Replies: 82
Views: 32,762
we could definitely DIY the process, the...
we could definitely DIY the process, the limitation being the bandwidth demand for any lab to do so. again this could be solved by using fedex-ing harddisks, but again who wants to take time in a lab...
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Forum: Bioinformatics
02-17-2011, 12:01 PM
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Replies: 14
Views: 3,515
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Forum: Bioinformatics
02-17-2011, 10:20 AM
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Replies: 14
Views: 3,515
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Forum: Bioinformatics
02-17-2011, 09:25 AM
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Replies: 14
Views: 3,515
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Forum: General
02-16-2011, 06:28 AM
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Replies: 13
Views: 10,764
K,
You are right about the RNA, but the bait...
K,
You are right about the RNA, but the bait design should be different. Nonetheless, it always bothered me when expression profiling involves amplification steps...
Wasn't there a protocol...
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Forum: General
02-16-2011, 03:10 AM
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Replies: 13
Views: 10,764
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Forum: Introductions
02-15-2011, 12:18 PM
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Replies: 4
Views: 1,404
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Forum: Bioinformatics
02-14-2011, 09:32 AM
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Replies: 82
Views: 32,762
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Forum: Bioinformatics
02-14-2011, 08:20 AM
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Replies: 5
Views: 1,794
Never had any chip-seq experience. But are the...
Never had any chip-seq experience. But are the peaks directly related to coverage? since chip-seq only selects a portion of the genome having high coverage (hence seeing higher peaks) is expected,...
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Forum: Bioinformatics
02-14-2011, 08:16 AM
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Replies: 1
Views: 1,237
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Forum: Bioinformatics
02-14-2011, 07:23 AM
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Replies: 3
Views: 2,398
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Forum: Introductions
02-14-2011, 06:10 AM
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Replies: 4
Views: 1,404
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Forum: Bioinformatics
02-14-2011, 06:07 AM
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Replies: 1
Views: 1,823
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Forum: Illumina/Solexa
02-13-2011, 10:16 PM
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Replies: 5
Views: 3,260
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Forum: Illumina/Solexa
02-13-2011, 06:25 PM
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Replies: 5
Views: 3,260
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Forum: Illumina/Solexa
02-13-2011, 12:17 PM
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Replies: 5
Views: 3,260
I never worked with Illumina's exome kit (only...
I never worked with Illumina's exome kit (only Sureselect) but sounds like the capture step failed due to the elution buffer, the bait never binded with the DNA targets. Or if it did, the AMPURE step...
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Forum: Bioinformatics
02-12-2011, 07:30 PM
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Replies: 1
Views: 2,157
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Forum: Literature Watch
02-12-2011, 03:53 AM
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Replies: 5
Views: 2,715
I always felt curious about job security for...
I always felt curious about job security for bioinformaticians in a lab that does sequence analysis. First of all, it's not an industrial environment where programmers could be promoted to become...
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Forum: Bioinformatics
02-11-2011, 06:14 PM
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Replies: 17
Views: 7,859
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Forum: Bioinformatics
02-11-2011, 03:58 AM
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Replies: 2
Views: 2,948
according to your needs. if you got to dataset...
according to your needs. if you got to dataset being merged with different RG but are really the same you could modify it to become the same. if you got 2 dataset with same RG but actually different...
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