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Forum: Sample Prep / Library Generation 03-19-2014, 07:42 AM
Replies: 4
Views: 3,586
Posted By ZWB
We find that the primary cause of concatamers...

We find that the primary cause of concatamers (assuming the RNA is abundant and of high quality) is over amplification in the final PCR step. I would try decreasing the number of PCR cycles....
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