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Forum: Illumina/Solexa 10-07-2011, 07:16 AM
Replies: 1
Views: 4,581
Posted By kthai
Yes, I completely agree with you. When I prepare...

Yes, I completely agree with you. When I prepare my ChIP-Seq libraries, the input and IP concentrations are the same to reduce any biases, especially during the amplification stage.
Forum: General 08-08-2011, 06:04 AM
Replies: 70
Views: 93,388
Posted By kthai
Hi James, Please add the MIT Biomicro Center...

Hi James,

Please add the MIT Biomicro Center to the map. We are located on 31 Ames St., Cambridge, MA.

We have 1 HiSeq 2000, 1 GAIIx, 2 GAII.

Thanks!
Forum: Illumina/Solexa 04-14-2011, 08:09 AM
Replies: 4
Views: 4,161
Posted By kthai
Have you looked into the KAPA enzymes?

Have you looked into the KAPA enzymes?
Forum: Illumina/Solexa 04-01-2011, 11:21 AM
Replies: 0
Views: 2,343
Posted By kthai
V3 PhiX

Hi all,

Has anyone used the new PhiX yet? If yes, did you receive positive results? I am trying to figure out if this new PhiX will somehow improve our long multiplexed runs in respect to our...
Forum: Illumina/Solexa 03-31-2011, 05:37 AM
Replies: 3
Views: 3,687
Posted By kthai
We typically load at 8pM, but this loading...

We typically load at 8pM, but this loading concentration may change depending on how multiplexed each lane is.

Also, it is important to note that this "8pM" loading concentration may vary for...
Forum: Illumina/Solexa 03-09-2011, 11:05 AM
Replies: 3
Views: 3,687
Posted By kthai
For the HiSeq, I have gotten great results when I...

For the HiSeq, I have gotten great results when I aim for the 700,000 K/mm^2 cluster density off of the first base report (usually about 90-120 million reads pass filter). I have had some luck with...
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