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Forum: Illumina/Solexa 01-06-2017, 08:08 AM
Replies: 23
Views: 7,096
Posted By pmiguel
Sure, those metrics would consistent with the...

Sure, those metrics would consistent with the start of a good run.

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Phillip
Forum: Illumina/Solexa 12-20-2012, 10:35 AM
Replies: 23
Views: 7,096
Posted By pmiguel
Yes. You want to remove the larger...

Yes.

You want to remove the larger fragments, you mean? You could do an Ampure upper cut, right?

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Phillip
Forum: Illumina/Solexa 12-19-2012, 11:33 AM
Replies: 23
Views: 7,096
Posted By pmiguel
We will likely spike in genomics libraries of...

We will likely spike in genomics libraries of some sort. That way some useful data is generated from the genomic 'ballast' library.

So you are saying that the quality values assigned are...
Forum: Illumina/Solexa 12-19-2012, 03:39 AM
Replies: 23
Views: 7,096
Posted By pmiguel
Rick, What? We did spike in phiX! We...

Rick,
What? We did spike in phiX!
We ostensibly spiked in 1% phiX -- but it turned out that only 0.3% of the reads derived from phiX.
phiX gives the % perfect read plot.

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Phillip
Forum: Illumina/Solexa 12-17-2012, 11:59 AM
Replies: 23
Views: 7,096
Posted By pmiguel
No, an RNA seq library. We will be doing...

No, an RNA seq library.

We will be doing some 16S fairly soon.

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Phillip
Forum: Illumina/Solexa 12-17-2012, 04:53 AM
Replies: 23
Views: 7,096
Posted By pmiguel
Thumbs up MiSeq best result?

I am calling out all who think they have good MiSeq runs. Gaze at the perfection of this run and weep at the inadequacy of your wretched instruments:
...
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