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Forum: Illumina/Solexa 03-01-2018, 08:07 PM
Replies: 7
Views: 1,763
Posted By nucacidhunter
It depends on the required coverage. If up to...

It depends on the required coverage. If up to 100M reads will give enough coverage then Nextera XT is better options. MDA normally will result in fusions which could be detrimental to your experiment.
Forum: Illumina/Solexa 11-17-2014, 05:24 PM
Replies: 7
Views: 1,763
Posted By nucacidhunter
I have had good results by cleaning up with zymo...

I have had good results by cleaning up with zymo gDNA clean and concentrater. One option to increase yield would be to elute with warm (55-60 C) Tris pH8 (Qiagen EB buffer) and incubate at the same...
Forum: Illumina/Solexa 11-13-2014, 11:55 PM
Replies: 7
Views: 1,763
Posted By nucacidhunter
MDA introduces lots of artefacts and I would...

MDA introduces lots of artefacts and I would consider it as a last resort. I have seen manufacturers results with E. coli DNA having only 70% of reads mapping to reference after MDA. Nextera XT does...
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