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Forum: Illumina/Solexa 05-13-2018, 05:49 PM
Replies: 14
Views: 3,107
Posted By chuanwu
What is the loading concentration ( ng/ul) you...

What is the loading concentration ( ng/ul) you got based on tape station?
Forum: Illumina/Solexa 05-11-2018, 05:27 PM
Replies: 14
Views: 3,107
Posted By chuanwu
ikripp, have you had a successful run recently?...

ikripp, have you had a successful run recently? What is your loading concentration with how much % of PhiX? Thanks
Forum: Sample Prep / Library Generation 05-04-2018, 12:56 PM
Replies: 13
Views: 3,002
Posted By chuanwu
Hi DPVT, Thank you for your reply. How did you...

Hi DPVT,
Thank you for your reply. How did you do 10,000 or 20,000 times dilution of your samples? Did you try two consecutive 1/100 dilutions or more than 2 consecutive dilutions? Such as 50X,...
Forum: General 05-04-2018, 09:07 AM
Replies: 6
Views: 3,227
Posted By chuanwu
Thank you for letting me know that qPCR quant is...

Thank you for letting me know that qPCR quant is more reliable.
Forum: Sample Prep / Library Generation 05-03-2018, 06:48 PM
Replies: 13
Views: 3,002
Posted By chuanwu
Hi, Did you believe the concentrations...

Hi,

Did you believe the concentrations according to the qPCR or the qubit HS concentration?
Thanks
Forum: General 05-03-2018, 05:44 PM
Replies: 6
Views: 3,227
Posted By chuanwu
LabChip GX

Did anyone used this to quant their amplicon library? Is that reliable? Whether the results are consistent with that measured by qPCR? Thanks
Forum: Illumina/Solexa 05-03-2018, 05:25 PM
Replies: 14
Views: 3,107
Posted By chuanwu
All the system checks passed. The only issue...

All the system checks passed.
The only issue for me why the quant of library measured by KAPA qPCR, Qubit, BioAnalyzer and LabChip GX are not consistent with each other. Which platform is reliable?...
Forum: Illumina/Solexa 05-02-2018, 05:40 PM
Replies: 14
Views: 3,107
Posted By chuanwu
I had the same issue twice in a row at Miseq...

I had the same issue twice in a row at Miseq platform. How did you guys resolve the problem. I called Illumina and they just questioned a lot about my library.

Thanks
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