Forum: RNA Sequencing
05-26-2014, 08:01 PM
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Replies: 1
Views: 4,564
Paired design versus unpaired design in DESeq2
Dear all,
I am assessing differential expression between tumour and normal gland samples from the same subjects using DESeq2 and have run into a problem using the paired design.
The dataset...
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Forum: Illumina/Solexa
04-15-2014, 08:13 PM
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Replies: 58
Views: 42,540
Hi Tom and Mike,
Thank you for your swift...
Hi Tom and Mike,
Thank you for your swift reply.
Tom, I really like your set-up with the internal standard as a competitor, already when I first read your paper a while ago. But we were at that...
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Forum: Illumina/Solexa
04-15-2014, 06:56 PM
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Replies: 58
Views: 42,540
Targeted RNA assay
Hi,
Again reviving the topic...
We are running a targeted RNA assay on our Miseq, and also have that issue with the carry-over of previously run libraries. We have two sets of about 40 targets...
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Forum: RNA Sequencing
12-03-2013, 05:40 PM
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Replies: 2
Views: 3,295
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Forum: Literature Watch
11-26-2013, 01:21 PM
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Replies: 3
Views: 5,865
Interesting!
Hi Tom,
I really enjoyed reading your paper! You touch on a couple of very important issues present in targeted RNA-sequencing.
I am currently working on a targeted RNA-sequencing assay, and am...
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Forum: RNA Sequencing
11-24-2013, 08:21 PM
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Replies: 2
Views: 3,295
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Forum: Introductions
11-20-2013, 09:41 PM
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Replies: 0
Views: 917
Hi!
Hi Everyone,
About a month ago I started working on a project involving targeted RNA sequencing on a Miseq/Hiseq. After numerous succesful visits to SEQanswers I finally decided to sign up and...
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