Forum: Illumina/Solexa
04-09-2010, 12:36 PM
|
|
Replies: 32
Views: 24,065
|
|
Forum: Illumina/Solexa
03-26-2010, 11:57 AM
|
|
Replies: 21
Views: 16,073
|
|
Forum: Illumina/Solexa
03-26-2010, 11:52 AM
|
|
Replies: 9
Views: 5,152
Same here
Looking for Library prep automation to increase throughput and eliminate variations caused by different users. In addition to the automation mentioned above from BIG_SNP, are there any other new...
|
|
Forum: Illumina/Solexa
01-27-2010, 08:39 AM
|
|
Replies: 74
Views: 397,717
So are most buffers if you ask qiagen or...
So are most buffers if you ask qiagen or invitrogen, but we are all smart people here, right? It is either photo or chemical cleavage and looking at all the steps in the sequencing process there...
|
|
Forum: Illumina/Solexa
01-13-2010, 08:42 AM
|
|
Replies: 14
Views: 17,303
|
|
Forum: Illumina/Solexa
01-12-2010, 07:27 PM
|
|
Replies: 14
Views: 17,303
Illumina's new sequencer: HiSeq 2000
Wow GAII just came out last year, they just now got the reagents QC under control after their SNAFU and now this. . .
http://www.illumina.com/systems/hiseq_2000.ilmn
How different can this...
|
|
Forum: Illumina/Solexa
12-10-2009, 01:53 PM
|
|
Replies: 1
Views: 6,368
Minimum size of DNA insertion fragments
I have small DNA fragments (avg 44 nt) that I would like to sequence but this is out of the recommended range of the GAII. I understand the limits of larger fragments (limited cluster density/large...
|
|
Forum: General
12-08-2008, 09:33 AM
|
|
Replies: 0
Views: 2,814
Fluidigm can help with sequencing?
I have read in various places (mostly their literature) that Fluidigm "eliminates costly titration steps" in next generation sequencing. I am unclear exactly how their technology really helps. Are...
|
|
Forum: The Pipeline
10-22-2008, 10:05 AM
|
|
Replies: 0
Views: 9,942
Oxford Nanopore
For originality this is the first 3rd gene sequencing by destruction. An exonuclease feeds cleaved bases into a modified hemolysin, while the potential is monitored. According to Oxford modified...
|
|
Forum: The Pipeline
10-22-2008, 08:40 AM
|
|
Replies: 0
Views: 3,923
NabSys 3rd gen
NabSys uses nanopores, combined with positional information obtained from sequential hybridization of a library of hexamers, to build sequence.
http://www.nabsys.com/
|
|
Forum: The Pipeline
10-22-2008, 08:29 AM
|
|
Replies: 1
Views: 6,426
Another company with deep pockets ponies up for...
Another company with deep pockets ponies up for 3rd gen. This looks like a hybrid between PacBio (single molecule, flurophore on the PO4, monitor incorporation) and Helicos (oligo array, visualize,...
|
|
Forum: Introductions
10-21-2008, 12:06 PM
|
|
Replies: 0
Views: 1,381
Hello All
I am a scientist working on 3rd gen nanopore sequencing technology. I work on mostly the upstream biochemistry, but as of late I have been dealing with novel technologies, instrumentation, and IP as...
|
|
Forum: Illumina/Solexa
10-16-2008, 09:05 AM
|
|
Replies: 74
Views: 397,717
Fluorophore removal
How does Illumina remove the fluorophore? They can't use AgNO3 (like SOLiD ) because of no Thio backbone. Are they using the Columbia IP that Intelligent BioSystems is using? ...
|
