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Forum: SOLiD 12-04-2012, 06:15 AM
Replies: 25
Views: 9,209
Posted By SeqTruth
Library/adapter compatibility

Can anyone tell me if Wildfire will work with standard SOLiD libraries, or even better, what the Wildfire adapter sequences are?
Forum: General 08-19-2012, 03:54 AM
Replies: 26
Views: 17,278
Posted By SeqTruth
Hi All, I have also been curious about...

Hi All,

I have also been curious about whether it's possible to use TruSeq or other 3rd party lib prep methods in conjunction with SureSelect. I understand the importance of using the appropriate...
Forum: Illumina/Solexa 02-25-2011, 03:27 AM
Replies: 1
Views: 1,824
Posted By SeqTruth
TruSeq "Oligo-only" kits?

I was wondering whether anyone knows whether Illumina is offering so-called "oligo-only" kits for making TruSeq indexed libraries, or whether they might be planning to?

I have looked around on...
Forum: Illumina/Solexa 02-01-2011, 12:20 AM
Replies: 38
Views: 58,359
Posted By SeqTruth
Ok, so somebody can maybe post those pdfs on a...

Ok, so somebody can maybe post those pdfs on a website somewhere, with some obvious search terms associated, and anyone with access to Google can find and download them; a minor inconvenience for...
Forum: Illumina/Solexa 01-12-2011, 06:09 AM
Replies: 13
Views: 5,412
Posted By SeqTruth
I had a similar idea: If one printed the...

I had a similar idea:

If one printed the capture/amplification oligos onto the flowcell, then it might be possible to perform a sort of sequence capture/enrichment in situ on the flowcell before...
Forum: Illumina/Solexa 01-12-2011, 05:54 AM
Replies: 35
Views: 45,312
Posted By SeqTruth
In case this is of interest to anyone, I believe...

In case this is of interest to anyone, I believe I have most of the TruSeq index sequences:

I stumbled on a screenshot in one of their tech bulletins that confirmed some preliminary sequence...
Forum: Illumina/Solexa 11-09-2010, 01:30 AM
Replies: 38
Views: 58,359
Posted By SeqTruth
Does anyone have explicit, detailed information...

Does anyone have explicit, detailed information about the new TruSeq adaptor sequences and structures?
Forum: Illumina/Solexa 11-07-2010, 11:40 PM
Replies: 3
Views: 3,927
Posted By SeqTruth
Thanks, GW_OK. I was not even aware that people...

Thanks, GW_OK. I was not even aware that people use different NaOH concentrations. Would you care to elaborate at all?

Does anyone have any insight into how any of those things on my list impact...
Forum: Illumina/Solexa 11-05-2010, 08:32 AM
Replies: 3
Views: 3,927
Posted By SeqTruth
Question Factors affecting cluster density

Apart from the obvious factor -- library concentration -- I was wondering what other variables affect cluster density. In other words, if one were to use qPCR for library quantification and load all...
Forum: 454 Pyrosequencing 07-09-2010, 12:27 AM
Replies: 4
Views: 8,659
Posted By SeqTruth
Thanks Marzanna! That has cleared up a number of...

Thanks Marzanna! That has cleared up a number of points of confusion for me :)
Forum: 454 Pyrosequencing 07-08-2010, 09:17 AM
Replies: 4
Views: 8,659
Posted By SeqTruth
FLX vs Titanium, Lib-L vs Lib-A, Amplicon Libraries = confusion

Hi All,

I'm hoping one or more of you can help to clarify some issues for me, and/or confirm whether I understand things correctly. There is extensive and detailed information about the adaptor...
Forum: General 04-17-2009, 07:14 AM
Replies: 2
Views: 5,943
Posted By SeqTruth
Adapter sequences

I'm looking for the sequences of both the 454 FLX and the Titanium library adapters. I'd also be interested to know the adapter sequences of any of the other platforms.

Thanks!
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