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Forum: Bioinformatics 10-28-2013, 07:55 AM
Replies: 2
Views: 1,273
Posted By BugSeq
I would recommend using filtering programs that...

I would recommend using filtering programs that keep track of the paired end relationship and separates them into another file when the mate doesn't meet your filtering requirements. I have had good...
Forum: Bioinformatics 10-28-2013, 07:18 AM
Replies: 5
Views: 2,268
Posted By BugSeq
I would recommend using filtering programs that...

I would recommend using filtering programs that keep track of the mate pair relationship and separates them into another file when the mate doesn't meet your filtering requirements. I have good luck...
Forum: Bioinformatics 10-24-2013, 12:26 PM
Replies: 1
Views: 1,804
Posted By BugSeq
SOAPec Segmentation Fault

Hello all. I am trying to use SOAPec KmerFreq_AR to filter and correct my reads before assembly. It seems to work great when the option -s is not set. The -s flag sets the space seed and turns on the...
Forum: Pacific Biosciences 09-19-2013, 12:23 PM
Replies: 0
Views: 1,954
Posted By BugSeq
LSC HC transformation question

I am pretty new to NGS and PacBio in particular, so I may be missing something fundamental. My question is regarding the LSC PacBio correction algorithm. In figure 1 of the below paper, it is briefly...
Forum: RNA Sequencing 02-13-2012, 09:31 AM
Replies: 0
Views: 1,379
Posted By BugSeq
one transcript many genes "chimeric"

The problem:
I am trying to assemble the transcriptome of Belgica the antarctic midge. Some of our assembled transcripts are much larger than they should be, and they contain multiple genes...
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