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Forum: Pacific Biosciences 02-10-2017, 04:18 AM
Replies: 7
Views: 2,106
Posted By naleph
The genome is Circular

The genome is Circular
Forum: Pacific Biosciences 02-10-2017, 02:42 AM
Replies: 7
Views: 2,106
Posted By naleph
Sure! here it is: http://imgur.com/yCvlMG1 ...

Sure! here it is:

http://imgur.com/yCvlMG1

Also the organism doesn't have a plasmid.
Thanks for your help.
Forum: Pacific Biosciences 02-09-2017, 02:50 AM
Replies: 7
Views: 2,106
Posted By naleph
I had thought of this scenario but duplication of...

I had thought of this scenario but duplication of almost a quarter of the genome is highly unlikely and illumina sequencing produces even coverage across the genome. Is it possible that a large...
Forum: Pacific Biosciences 02-08-2017, 09:10 AM
Replies: 7
Views: 2,106
Posted By naleph
Uneven coverage across scaffold

Hi,

I have run the SMRT-Portal RS_HGAP_Assembly.2 protocol.
I am getting a single whole chromosome assembled (~4.4Mb) but there is uneven coverage across the scaffold.

See Figure:...
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