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Forum: Illumina/Solexa 12-05-2019, 01:53 PM
Replies: 6
Views: 2,395
Posted By saimonara
Hi kcchan, yes, I updated to v2.20 this...

Hi kcchan,

yes, I updated to v2.20 this morning but saw the same behavior later in the day. However, when I reran bcl2fastq I got the correct number of reads. I think it might be something that...
Forum: Illumina/Solexa 12-05-2019, 09:44 AM
Replies: 6
Views: 2,395
Posted By saimonara
I'm experiencing this problem as well!...

I'm experiencing this problem as well! Unfortunately, I just notice this error and a third of the ~40 runs we've done have the problem. The runs are not sequential and there were no software updates...
Forum: Bioinformatics 05-13-2016, 12:43 PM
Replies: 1
Views: 879
Posted By saimonara
Any ideas? Anyone?

Any ideas? Anyone?
Forum: Bioinformatics 05-12-2016, 08:02 AM
Replies: 1
Views: 879
Posted By saimonara
Lightbulb Comparing multiple nuclear capture probe sets

Hi,

I'm interested in doing HybSeq and I designed a few sets of baits/probes to do capture sequencing of hundreds of nuclear loci in ~120 species of a plant genus. All species are closely related...
Forum: General 02-13-2014, 03:58 PM
Replies: 13
Views: 6,123
Posted By saimonara
Are you setting the reactions on ice? I find that...

Are you setting the reactions on ice? I find that setting everything on ice and then going to a preheated thermocycler reduces unspecific annealing. I always add a first step in my thermal profiles...
Forum: General 02-13-2014, 12:40 PM
Replies: 13
Views: 6,123
Posted By saimonara
What type of reagents are you using? I have...

What type of reagents are you using? I have experience with long PCR with fragments that ranged from 5kb-12kb. When we first started trying to amplify these however, we were using commercially...
Forum: Illumina/Solexa 02-12-2014, 06:47 PM
Replies: 2
Views: 3,364
Posted By saimonara
Is the maximum recommended size 1kb in order to...

Is the maximum recommended size 1kb in order to avoid cluster overlap during bridge amplification? Have people successfully sequenced longer fragments?

The majority of my amplicons are between...
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