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Forum: Sample Prep / Library Generation 05-27-2010, 09:35 AM
Replies: 11
Views: 5,264
Posted By Apratim
Thanks for the explanation. I can understand when...

Thanks for the explanation. I can understand when you are polishing a 3' overhang you can remove extra base by exonuclease activity and then fill-it up and hopefully incorporate a biotinylated one....
Forum: Sample Prep / Library Generation 05-27-2010, 08:43 AM
Replies: 11
Views: 5,264
Posted By Apratim
I am also after "Home-made" mate-pair protocol

I am also wondering about the biotinylation step. Also when you pre-incubate with T4 DNA polymerase, T4 PNK, and Klwnow in presence of dNTPS will you not polish/fill the the ends? Then how the...
Forum: Introductions 10-07-2009, 04:58 AM
Replies: 373
Views: 169,088
Posted By Apratim
Greetings to members

Hi, I just joined the University of Exeter and planning to do RNASeq work on rice-blast fungus (Magnaporthe). The technique and analysis are quite new to me; so please excuse my basic questions....
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