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Forum: RNA Sequencing 08-16-2012, 08:35 PM
Replies: 27
Views: 59,962
Posted By weijenc
Problem in grooming

Thanks for the reply to my previous post.

I have been trying to work with the paired-end dataset (SRR131208, two files). After grooming (solexa to fastq sanger), however, quality values are...
Forum: RNA Sequencing 08-13-2012, 06:02 AM
Replies: 27
Views: 59,962
Posted By weijenc
Trimming Paired-End Data

Hello,

So if I use quality value < 20 to trim my Illumina dataset, which contains paired-end 100 bp sequencing reads, would both reads on the same pair be removed should one of them have a base...
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