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Forum: Sample Prep / Library Generation 03-01-2011, 07:50 PM
Replies: 3
Views: 5,212
Posted By omnivore
Unhappy Non-specific band after gel purification of PCR products

I'm trying to purify my PCR products (obtained by using barcoded fusion primes) by gel purification to increase the quality of amplicon pyrosequencing.

My problem is that non-specific band of...
Forum: 454 Pyrosequencing 10-13-2010, 04:33 AM
Replies: 6
Views: 4,994
Posted By omnivore
Thanks for replies. I'm sure about DNA sizes...

Thanks for replies.

I'm sure about DNA sizes (0.7% agarose gel was used).

I think that bead beating step (introduced for some reasons) was the cause of DNA shearing.
Forum: 454 Pyrosequencing 10-12-2010, 04:29 PM
Replies: 6
Views: 4,994
Posted By omnivore
Thanks for your interest. I attach a new gel...

Thanks for your interest.

I attach a new gel image (marker sizes are indicated).
Forum: 454 Pyrosequencing 10-12-2010, 12:03 AM
Replies: 6
Views: 4,994
Posted By omnivore
Unhappy Can I use sheared genomic DNA as starting material of bacterial genome sequencing?

I am planning to acquire draft genome sequence of a bacterial strain by using GS FLX titanium sequencer. My question is whether initially sheared genomic dna can be safely used for genome sequencing...
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