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Forum: Bioinformatics 03-02-2015, 08:24 AM
Replies: 15
Views: 9,088
Posted By jparsons
In my opinion, ERCC spike-ins are uniquely...

In my opinion, ERCC spike-ins are uniquely situated to determine the mRNA:totalRNA ratio between samples, and are best used when you expect that the ratio is NOT equal. The Nature Biotech paper...
Forum: Bioinformatics 01-07-2014, 09:16 AM
Replies: 15
Views: 9,088
Posted By jparsons
The DESeq size factors assume that most things...

The DESeq size factors assume that most things will be 1:1, so the 1:1 sub pool would be a good fit.

That said, it's data analysis so there's nothing stopping you from doing it both ways - I'd be...
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