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Forum: Bioinformatics 01-27-2017, 12:12 AM
Replies: 94
Views: 19,465
Posted By seq4franken
multiple fastq.gz for Tadpole

Hello,
is it possible to specify multiple fastq.gz files (paired-end) as input for Tadpole?
I try to find a way to circumvent decompression and concatenation.

Thanks for any suggestion!
Alfons
Forum: RNA Sequencing 11-07-2012, 10:14 PM
Replies: 9
Views: 1,957
Posted By seq4franken
I run Velvet/Oases directly from command line...

I run Velvet/Oases directly from command line successfully. I also created an .afg file. The issue with Geneious Server is that there is no Oases plugin available for Geneious (although a Velvet...
Forum: RNA Sequencing 11-06-2012, 10:08 PM
Replies: 9
Views: 1,957
Posted By seq4franken
Hello, thanks a lot for your replies. ...

Hello,

thanks a lot for your replies.

@AdrianP: Geneious Server is also from Biomatters (who makes Geneious); more details are on their website. Our server has 192 GB RAM and 24 CPUs. So, we...
Forum: RNA Sequencing 10-14-2012, 11:12 PM
Replies: 9
Views: 1,957
Posted By seq4franken
Thanks for your post. I realized that there are...

Thanks for your post. I realized that there are 'abundant' tools available and I read the description of most of them. Unfortunately, I'm not an Linux expert, so most of them are not really suited to...
Forum: RNA Sequencing 10-12-2012, 04:35 AM
Replies: 9
Views: 1,957
Posted By seq4franken
RNAseq quantification - Velvet assembler

Hi

we are using the Velvet assembler plugin in Geneious Server and were able to generate ca. 50,000 contigs from ca. 120 Mio paired reads (Illumina).

Unfortunately, the Velvet Geneious plugin...
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