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Forum: Illumina/Solexa 11-21-2012, 09:59 AM
Replies: 1
Views: 4,222
Posted By thdybwf
Question How much PhiX in MiSeq run?

Hello all -

Our lab is about to run a RADseq library on an Illumina MiSeq. It consists of multiplexed individuals with semi-random fragments from across the genome.

We're not sure how much...
Forum: Illumina/Solexa 10-24-2012, 07:05 PM
Replies: 2
Views: 5,256
Posted By thdybwf
Question HiSeq Library compatible with MiSeq?

Hello all -

We've prepared our first library following a protocol originally for the Illumina Genome Analyzer. It's out for QC now and I'm keeping my fingers crossed. I'm now wondering, though,...
Forum: Sample Prep / Library Generation 09-18-2012, 06:36 PM
Replies: 5
Views: 5,308
Posted By thdybwf
Unhappy Library invisible on gel, despite Nanodrop results. Gel extraction problem?

I'm having some difficulty with my very first Illumina library prep, and was hoping someone might point out any obvious errors I'm committing in my ignorance. I'm using a protocol that involves size...
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