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Forum: Illumina/Solexa 01-06-2017, 09:28 PM
Replies: 68
Views: 18,348
Posted By Shimul
Hello there, I am running my bacterial...

Hello there,

I am running my bacterial library using Miseq V3. The Q30 is now 83.1% after 250 cycles, yesterday it was 96% after 70 cycles. But rest of the parameters are consistent: clusters PF...
Forum: Illumina/Solexa 01-06-2017, 09:09 PM
Replies: 23
Views: 7,092
Posted By Shimul
The Q30 is now 83.1% after 250 cycles, yesterday...

The Q30 is now 83.1% after 250 cycles, yesterday it was 96% after 70 cycles. But rest of the parameters are consistent: clusters PF 91.4%, cluster density 1092K/mm^2, which are the same as yesterday....
Forum: Illumina/Solexa 01-06-2017, 07:42 AM
Replies: 23
Views: 7,092
Posted By Shimul
Hi All, I am finally running of my bacterial...

Hi All,

I am finally running of my bacterial pooled DNA library (which I made using Nextera XT) on the Miseq using MiSeq V3. I have checked the machine now and it shows that after 70 cycles, the...
Forum: RNA Sequencing 01-06-2017, 07:31 AM
Replies: 6
Views: 1,603
Posted By Shimul
jdk787

Thanks a lot Josh!

Cheers,

Shimul
Forum: RNA Sequencing 01-06-2017, 06:34 AM
Replies: 6
Views: 1,603
Posted By Shimul
Hello, I am finally running of my sample on...

Hello,

I am finally running of my sample on the Miseq using MiSeq V3. I have checked the machine now and it shows that after 70 cycles, the >=Q30 is 96%, cluster passing filter 91.4%, and the...
Forum: Illumina/Solexa 01-06-2017, 06:28 AM
Replies: 12
Views: 5,946
Posted By Shimul
Hi There, I am running my bacterial sample...

Hi There,

I am running my bacterial sample using MiSeq V3. After 70 of 600 cycles, it showed cluster density is 1092K/mm, cluster passing filter 91.4% and the >=Q30 is 96%. Do you think that the...
Forum: RNA Sequencing 01-01-2017, 09:59 PM
Replies: 6
Views: 1,603
Posted By Shimul
Thanks a lot @ nucacidhunter

Thanks a lot @ nucacidhunter
Forum: RNA Sequencing 01-01-2017, 07:23 PM
Replies: 6
Views: 1,603
Posted By Shimul
Library insert size

Hi There,

I am aiming to sequence bacterial genome with NexteraXT 300bp paired-end reads. I have made my library and all the fragments size (including the adapter) are ranging from 200-650 (I...
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