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Forum: Illumina/Solexa 07-21-2017, 01:01 PM
Replies: 13
Views: 3,264
Posted By ianakiev
Illumina tech support may have to refresh their...

Illumina tech support may have to refresh their training. If you don't use system (HiSeq 2500) for more than 10 days you are supposed to do maintenance wash aka. tween + ProClin 300 and then shut...
Forum: Illumina/Solexa 07-20-2017, 02:53 PM
Replies: 7
Views: 1,055
Posted By ianakiev
The sequences in green colors are needed for...

The sequences in green colors are needed for hybridization to the flow cell primers. That is what I meant. Cheers
Forum: Illumina/Solexa 07-20-2017, 01:02 PM
Replies: 13
Views: 3,264
Posted By ianakiev
ProClin 300 is another name for DowChemical...

ProClin 300 is another name for DowChemical product called KATHON
Or at least that is what I understand. Correct me if I'm wrong.
Forum: Illumina/Solexa 07-19-2017, 10:21 AM
Replies: 7
Views: 1,055
Posted By ianakiev
You need these "Long" primers in order to be able...

You need these "Long" primers in order to be able to cluster/sequence. Cheers
Forum: Illumina/Solexa 07-14-2017, 09:54 AM
Replies: 6
Views: 848
Posted By ianakiev
Brian, thanks for your input. I will spike in...

Brian, thanks for your input. I will spike in some e-coli libraries that I already sequenced, however I still can fit in an experiment given the e.coli can be small fraction.
Thanks again.
Peter
Forum: Illumina/Solexa 07-14-2017, 09:31 AM
Replies: 6
Views: 848
Posted By ianakiev
Free NGS 1x50 SE rapid run

Hi All,

I have one Free Rapid 1x50 SE run to qualify my recently acquired HiSeq 2500. I thought it will be shame to waste it with PhX control, so I am open to hear your ideas and proposals for...
Forum: Introductions 07-14-2017, 09:19 AM
Replies: 0
Views: 528
Posted By ianakiev
NGS service Boston area

Hi Fellows NGS users,

I want to introduce myself. I am Peter. I have been in NGS development for quite some time. Started HERA Biosciences as CRO for NGS and for over two years I have been serving...
Forum: Illumina/Solexa 07-14-2017, 07:37 AM
Replies: 3
Views: 838
Posted By ianakiev
HiSeq startup

Try starting the sequencer first, then the computer. I guess Illumina put bunch of thumb drives (aka. flash drives) on the PCBs inside the instrument.

How much do you ask for HiSeq 2000?
cheers
Forum: Sample Prep / Library Generation 10-10-2015, 08:05 PM
Replies: 4
Views: 2,783
Posted By ianakiev
I have IntegenX Apollo 324 instrument, however I...

I have IntegenX Apollo 324 instrument, however I am not sure how to update scripts on it? P-suite experience anyone>?
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