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Forum: Illumina/Solexa 04-15-2020, 11:40 PM
Replies: 1
Views: 1,765
Posted By Saravanan
I think your library might be degraded. I elute...

I think your library might be degraded. I elute my library in Tris Low-EDTA buffer. It works well for me.
Forum: Illumina/Solexa 04-15-2020, 09:45 PM
Replies: 2
Views: 1,976
Posted By Saravanan
We had issue with low PF few months ago. We...

We had issue with low PF few months ago. We called the engineer to fix it. He cleaned the camera and other stuffs and now it's working fine.
Forum: Illumina/Solexa 04-15-2020, 12:31 AM
Replies: 1
Views: 1,238
Posted By Saravanan
I kept the reagent cartridge in water bath for...

I kept the reagent cartridge in water bath for almost 2 hr and it was OK.
The cluster density 1050 K/mm2 is in normal range.
I think the quality of your sample might not good that's why you got...
Forum: Illumina/Solexa 05-23-2019, 06:49 PM
Replies: 10
Views: 8,891
Posted By Saravanan
Thank you so much for your reply.

Thank you so much for your reply.
Forum: Illumina/Solexa 05-15-2019, 07:36 PM
Replies: 10
Views: 8,891
Posted By Saravanan
@thiNGS, I accidentally thawed the wrong Nextseq...

@thiNGS, I accidentally thawed the wrong Nextseq 500 reagent cartridges and refrozen it. I would like to know about your result. Is it worked fine?

Thank you
Saravanan
Forum: Sample Prep / Library Generation 08-28-2018, 06:30 PM
Replies: 20
Views: 24,782
Posted By Saravanan
TD buffer recipe

Could you share the TD buffer recipe. I couldn't find it.

Many thanks
Saravanan
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