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Forum: Illumina/Solexa 03-03-2011, 09:00 PM
Replies: 73
Views: 351,304
Posted By li zhang
Question pair-end sequence poplar transcriptome

Hi there,

I wanna do mRNA-seq on poplar, I review a bunch of literature without finding a single paper using pair-end sequencing, most of them use 454 sequencing. Does that mean pair-end...
Forum: RNA Sequencing 03-02-2011, 09:59 AM
Replies: 36
Views: 23,225
Posted By li zhang
I wanna examine transcriptome including novel...

I wanna examine transcriptome including novel transcripts, I am told microarray is not so sensitive
Forum: RNA Sequencing 02-04-2011, 10:12 PM
Replies: 36
Views: 23,225
Posted By li zhang
Thx, Tonybrooks. Actually we wanna detect which...

Thx, Tonybrooks. Actually we wanna detect which genes are active during a specific period and in a particular tissue, and identify these expressed genes which are responsible for a particular...
Forum: RNA Sequencing 02-02-2011, 11:07 AM
Replies: 36
Views: 23,225
Posted By li zhang
Hi, Im a beginner at sequencing. I have several...

Hi, Im a beginner at sequencing. I have several might silly questions. Are RNA-seq and transcritome sequencing the same thing and using the same protocol? Can paired-end squencing be adopted in...
Forum: Illumina/Solexa 01-29-2011, 07:26 PM
Replies: 73
Views: 351,304
Posted By li zhang
Thanks for the useful information

Thanks for the useful information
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