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Forum: General 07-13-2016, 11:14 AM
Replies: 0
Views: 1,031
Posted By kwalton
REDCap for a genomics facility

Has anyone implemented REDCap as a way to track samples as they go through the process(es) in a core? It looks like it can track samples to a point but I'm not sure I can add the information I need...
Forum: Sample Prep / Library Generation 03-10-2016, 02:25 PM
Replies: 7
Views: 2,801
Posted By kwalton
I do this protocol with sorted cells and...

I do this protocol with sorted cells and routinely use only 1ng of total RNA (according to the bioanalyzer pico chip- yes I know it's not quantitative but it's the only measure of concentration I can...
Forum: Illumina/Solexa 02-26-2016, 03:36 PM
Replies: 15
Views: 8,525
Posted By kwalton
2500 vs 4000

We are in a position to buy an Illumina. I have a lot of experience with the 2500 at my previous job, but have not run Illumina technology in ~2yrs. At my current position we are debating the 2500...
Forum: Sample Prep / Library Generation 03-06-2014, 11:40 AM
Replies: 0
Views: 1,543
Posted By kwalton
RNase III digestion time for Ion Total RNA-seq

We are doing poly A selection from 100ng of Total RNA. After building the libraries, the insert size looks to be around 100bp. We are wondering if the RNase III digestion is too long for such low...
Forum: Sample Prep / Library Generation 03-05-2014, 01:21 PM
Replies: 1
Views: 1,398
Posted By kwalton
Pippin vs. Egel

Hi all,
We are looking for opinions on whether you prefer the Pippin or E gel for size selection when doing NGS libraries. Has anyone compared them side by side? We are a low throughput lab with the...
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