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Forum: General 10-07-2017, 02:33 AM
Replies: 3
Views: 1,411
Posted By prolife
Yes I'll sequence 400M molecules. But if my...

Yes I'll sequence 400M molecules. But if my fragmented DNA is around 150 bp the difference compared single and paired is that in the latter I'll sequence all my fragments, isn't it?
Forum: General 10-06-2017, 02:20 PM
Replies: 3
Views: 1,411
Posted By prolife
reads number in single-end vs paired end seq

Hi all,

probably a stupid question but, i'm going to use NextSeq500 and preparing my sample with TruSeq Nano library prep kit.

Sequencing will be performed by the V2 kit, 75 cycles.

So, the...
Forum: Bioinformatics 07-31-2014, 03:09 AM
Replies: 39
Views: 3,100
Posted By prolife
Solved, thanks!

Solved, thanks!
Forum: Bioinformatics 07-31-2014, 01:48 AM
Replies: 39
Views: 3,100
Posted By prolife
Bowtie2 basic problem!

Hi

I'm new in the bioinformatic field and I have a basic problem

I have a read,in fastq format, ready to align on my reference genome (human genome), but I'm not able to do that!

I've...
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