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Forum: Sample Prep / Library Generation 05-22-2019, 05:06 AM
Replies: 0
Views: 366
Posted By Micro151086
Would you go ahead with RNA-seq?

Hello,

I have 27 samples of total RNA isolated from E. coli which (having passed all initial requirements) have failed QC prior to sequencing. We are using a HiSeq 2 x 150 bp reads. The RINs are...
Forum: General 05-22-2019, 01:24 AM
Replies: 3
Views: 640
Posted By Micro151086
Thank you for the reply. I've added some images...

Thank you for the reply. I've added some images that will hopefully shed some light on the situation. Many thanks!
Forum: General 05-21-2019, 11:27 AM
Replies: 3
Views: 640
Posted By Micro151086
High RIN low DV200?? Can anybody shed some light?

I've recently sent off E. coli RNA samples for RNA-seq. The company we have used for sequencing have replied and said all samples failed QC due to degradation. I am looking at the Tapestation values...
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