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Forum: Illumina/Solexa 07-27-2017, 12:25 AM
Replies: 2
Views: 567
Posted By F.Lazaro
Thanks! I will take it into account!

Thanks!

I will take it into account!
Forum: Sample Prep / Library Generation 07-26-2017, 03:25 AM
Replies: 3
Views: 1,235
Posted By F.Lazaro
Reducing costs in library preparation

Hello all,

I have read in other posts that is feasible to reduce volumes for library preparation when using the Nextera XT kit. Do you know if its also posible to reduce the volumes by a half when...
Forum: Illumina/Solexa 07-26-2017, 01:40 AM
Replies: 2
Views: 567
Posted By F.Lazaro
Miseq V2 Micro Yield

Hello everyone,

I'm planing to buy the Miseq V2 Micro kits for low throughput experiments. Illumina claims a yield of 4M reads. So, someone have experience using this kit? And if so, which is...
Forum: Illumina/Solexa 06-13-2017, 07:30 AM
Replies: 4
Views: 424
Posted By F.Lazaro
Thanks! That was my intention. To shear both...

Thanks!

That was my intention. To shear both genomes to almost the same fragment size and indexing each sample.
Regarding the proportion of bacterial genomes and viromes, is there any way to...
Forum: Illumina/Solexa 06-13-2017, 07:00 AM
Replies: 4
Views: 424
Posted By F.Lazaro
Question Sequence bacterial genome and amplicons on the same run

Hello everyone,

At the moment we are using Miseq to sequence both bacterial and viral genomes at very low throughput. I would like to know if there could be any problem in sequencing these very...
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