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Forum: Sample Prep / Library Generation 04-21-2012, 06:15 AM
Replies: 11
Views: 3,415
Posted By guz
I know there is miRNA present bc I've done qPCR...

I know there is miRNA present bc I've done qPCR on specific targets. I'm pretty sure you nailed with it by saying it is 5S, 5.8S and degraded RNA.

Thanks for your help :)

-Andy
Forum: Sample Prep / Library Generation 04-20-2012, 05:37 AM
Replies: 11
Views: 3,415
Posted By guz
Is this peak specific to brain or do you see it...

Is this peak specific to brain or do you see it across tissue types when using Trizol?

I wasn't certain this peak was attributed to small RNA species like pri-miRNA or tRNA because I didn't think...
Forum: Sample Prep / Library Generation 04-19-2012, 07:20 PM
Replies: 11
Views: 3,415
Posted By guz
Strange peak in total RNA extractions from brain tissue

We have extracted total RNA from frozen brain tissue. To extract RNA, we homogenized 30-50mg tissue with Trizol then added chloroform for phase separation and lastly did a buffer exchange using the...
Forum: Ion Torrent 03-21-2011, 10:34 AM
Replies: 14
Views: 11,282
Posted By guz
wildung, you should check out Ion's bulk breaking...

wildung, you should check out Ion's bulk breaking protocol. It is much quicker and far less tedious than spinning individual tubes.
Forum: 454 Pyrosequencing 11-12-2009, 10:20 AM
Replies: 28
Views: 9,226
Posted By guz
thank you jason i am very excited for the release

thank you jason i am very excited for the release
Forum: 454 Pyrosequencing 11-09-2009, 06:47 AM
Replies: 28
Views: 9,226
Posted By guz
Does anyone have the REM system in their lab that...

Does anyone have the REM system in their lab that does want to post about it? How does it work?
Forum: 454 Pyrosequencing 11-06-2009, 09:01 AM
Replies: 9
Views: 3,748
Posted By guz
We have also seen a crystallized precipitate...

We have also seen a crystallized precipitate after heating GC additive. I suggest spinning down the tube after heating and only removing GC additive that is found above the pellet. We have found GC...
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