Forum: Bioinformatics
07-11-2012, 06:10 PM
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Replies: 1
Views: 360
How to calculate gene expression level
Hi, everyone!
I have the gtf annotation for mouse genome and rna-seq mapping to genome result(sam/bam). I want to know whether a software could calculate the genes' expression level based on these...
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Forum: Bioinformatics
03-04-2012, 04:32 PM
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Replies: 1
Views: 1,795
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Forum: Bioinformatics
01-31-2012, 11:57 PM
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Replies: 2
Views: 455
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Forum: Bioinformatics
01-30-2012, 08:19 PM
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Replies: 2
Views: 455
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Forum: Bioinformatics
05-30-2011, 06:06 PM
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Replies: 8
Views: 1,221
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Forum: Bioinformatics
05-30-2011, 05:41 PM
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Replies: 8
Views: 1,221
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Forum: Bioinformatics
05-30-2011, 05:38 PM
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Replies: 20
Views: 5,186
improperly sorted SAM !
you have to revise...
improperly sorted SAM !
you have to revise the merge script manually. Please give the sam file a head.
for version cufflinks_1.0.2, the line 544, you can revise like that:
header = '''@SQ...
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Forum: Bioinformatics
05-30-2011, 04:56 PM
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Replies: 5
Views: 1,815
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Forum: Bioinformatics
05-30-2011, 07:24 AM
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Replies: 5
Views: 1,815
The test for cuffmerge
To whom it may concern,
Thanks for the excellent software. I have 8 mouse samples, and get the transcripts for each sample using cufflinks. When I use cuffmerge to merge each sample to one file, I...
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Forum: RNA Sequencing
12-15-2010, 04:42 PM
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Replies: 0
Views: 582
One question about Tophat
When I use Tophat to get the unqiue reads which just mapped to one location in the genome, I have any problems. When I get the sam file, I filter it with the criterion that the last column is...
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Forum: Illumina/Solexa
03-23-2010, 04:24 PM
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Replies: 1
Views: 1,645
Solexa history
Anyone could tell me the Solexa history? how many generations the Solexa has passed through?
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Forum: General
01-28-2010, 04:40 PM
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Replies: 12
Views: 2,313
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Forum: Introductions
01-28-2010, 04:24 PM
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Replies: 0
Views: 521
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