SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 13 of 13
Search took 0.00 seconds.
Search: Posts Made By: dorix
Forum: Illumina/Solexa 04-04-2019, 06:22 AM
Replies: 4
Views: 3,112
Posted By dorix
auzzie599, would you share your experience? The...

auzzie599, would you share your experience? The logic says it should not change the PCR yield (not taking into account differences between PPC/Nextera primers efficiency. Am I right?
Forum: De novo discovery 02-02-2017, 04:22 AM
Replies: 3
Views: 8,432
Posted By dorix
Hi, I have the same problem. Has anyone...

Hi,
I have the same problem. Has anyone managed to solve it?

Interesting fact is that I have managed to finish many samples with the same machine, mpi, settings, comparable complexity and...
Forum: Illumina/Solexa 10-17-2016, 11:11 PM
Replies: 18
Views: 7,429
Posted By dorix
The final answer for my problem is even more...

The final answer for my problem is even more complicated, as I found out that my actual library size is different, as T-shaped adapters cause higher migration of libraries, as it is shown in TruSeq...
Forum: Illumina/Solexa 10-17-2016, 11:04 PM
Replies: 13
Views: 5,082
Posted By dorix
The final answer for my problem is even more...

The final answer for my problem is even more complicated, as I found out that my actual library size is different, as T-shaped adapters cause higher migration of libraries, as it is shown in TruSeq...
Forum: Illumina/Solexa 10-17-2016, 09:55 AM
Replies: 18
Views: 7,429
Posted By dorix
Thank you for your response, Jessica. The shape...

Thank you for your response, Jessica. The shape seems fine to me, Gaussian type shifted towards higher lengths. I have also seen amplicon libraries without amplification, which has given me a sense...
Forum: Illumina/Solexa 10-17-2016, 09:41 AM
Replies: 13
Views: 5,082
Posted By dorix
jdk787, thank you for your resposne. Have you got...

jdk787, thank you for your resposne. Have you got any expirience with lower concentration from qPCR due to too short elongation time and how does it corresponds to amplifiaction on machine?
Forum: Illumina/Solexa 10-17-2016, 07:45 AM
Replies: 13
Views: 5,082
Posted By dorix
Hi, I have libraries of insert size of about...

Hi,
I have libraries of insert size of about 600bp. I'm quantifing them by qPCR. Those are amplification free libraries, so I gess I may have same fragments wihout adapters and so on. Unfortunatelly...
Forum: Illumina/Solexa 10-17-2016, 05:04 AM
Replies: 18
Views: 7,429
Posted By dorix
Hi, I have libraries of insert size of about...

Hi,
I have libraries of insert size of about 600bp. I'm quantifing them by qPCR. Those are amplification free libraries, so I gess I may have same fragments wihout adapters and so on. Unfortunatelly...
Forum: Illumina/Solexa 05-24-2016, 10:46 PM
Replies: 12
Views: 7,525
Posted By dorix
What do you mean? As Hyeshik Chang, we want...

What do you mean?

As Hyeshik Chang, we want to analyze homopolymeric sequences.
Forum: Illumina/Solexa 05-18-2016, 06:04 AM
Replies: 12
Views: 7,525
Posted By dorix
I've also got the answer from tech support which...

I've also got the answer from tech support which warned me that .cif files is not supported due to memory limitations. I wonder if you saved .cif files on machine or on external drive and if any...
Forum: Illumina/Solexa 05-17-2016, 03:44 AM
Replies: 12
Views: 7,525
Posted By dorix
Hi, We're planning to save .cif files from...

Hi,
We're planning to save .cif files from NextSeq sequencing. We have been warned that new chemistry is not compatible with it. Your comments seems promising, however I would like to be sure that...
Forum: Illumina/Solexa 07-21-2015, 10:48 PM
Replies: 10
Views: 7,237
Posted By dorix
Thanks a lot!

Thanks a lot!
Forum: Illumina/Solexa 07-21-2015, 04:47 AM
Replies: 10
Views: 7,237
Posted By dorix
Hi, I'm wondering if someone was saving cif...

Hi,
I'm wondering if someone was saving cif files from Miseq with new software.
I can't find file: MiSeqControlSoftware.Options.cfg in my MCS. Or maybe first change in...
Showing results 1 to 13 of 13

 


All times are GMT -8. The time now is 06:27 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO