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Forum: Illumina/Solexa 08-13-2015, 09:54 AM
Replies: 5
Views: 10,680
Posted By HESmith
Brian's analysis is correct (as usual). I should...

Brian's analysis is correct (as usual). I should have said that Illumina assigns a conservative (lower) quality score to those early cycles. And you can clearly see the reduced quality of the last...
Forum: Illumina/Solexa 08-13-2015, 04:30 AM
Replies: 5
Views: 10,680
Posted By HESmith
The first four cycles of data are used for...

The first four cycles of data are used for cluster calling, and also for establishing metrics (e.g., signal thresholds) for base-calling. Base-calls for the first cycles rely on a standard set of...
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