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Forum: RNA Sequencing 05-03-2012, 08:08 AM
Replies: 0
Views: 1,206
Posted By seqfan2011
Question Small RNA sequencing depth comparison to array or qPCR

Hi,

Has anyone came across small RNA-seq comparison study with array or qPCR? Such as how many millions reads would be needed to reach the senstivity of qPCR for detection of small RNA at CT value...
Forum: Illumina/Solexa 05-03-2012, 07:59 AM
Replies: 22
Views: 11,087
Posted By seqfan2011
Has anyone tried Truseq small RNA kit with <100ng...

Has anyone tried Truseq small RNA kit with <100ng total RNA input? We have some precious samples for microRNA study, but can't seem to find any kit on the market supporting such low input. Any...
Forum: General 05-01-2012, 01:33 PM
Replies: 0
Views: 1,451
Posted By seqfan2011
Smile small RNA sequencing with total RNA less than 100ng

Hi,

Does anyone have experience with small RNA sequencing of total RNA samples less than 100ng? We have been using Illumina small RNA kit for samples with 1ug, which is minimum input requirement...
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