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Forum: Sample Prep / Library Generation 11-04-2016, 01:33 AM
Replies: 8
Views: 1,129
Posted By NGSsequencer
IM just curious why you would want to do this? ...

IM just curious why you would want to do this? obviously unless this is a single cell.

miRNA size is 21bp, but the miRNA library size is 140-170nt+ depending on size. These libraries consist of...
Forum: Sample Prep / Library Generation 11-01-2016, 08:02 PM
Replies: 8
Views: 1,129
Posted By NGSsequencer
Currently there are no sequencing based methods...

Currently there are no sequencing based methods that allow you to use the same sample to simultaneously sequence both large and small RNA. Remember, different transcript sizes, which could yield...
Forum: Illumina/Solexa 10-15-2014, 05:55 AM
Replies: 1
Views: 678
Posted By NGSsequencer
Cost per base decrease over the last few years

Working on a case study for B-School. Since price for most testing has decreased at certain output levels, I was hoping the community might be able to provide me with some historical context

For...
Forum: General 07-27-2014, 05:35 AM
Replies: 1
Views: 1,531
Posted By NGSsequencer
Illumina Long ReadKit (Moleculo) on MiSeq

Has anyone taken the moleculo kit and run on the 250bp runs on the Miseq and tried the assembly after? I'd like to know if it would work. On the Illumina website only up to the 2500 is supported,...
Forum: 454 Pyrosequencing 05-06-2013, 07:03 PM
Replies: 1
Views: 858
Posted By NGSsequencer
Best 454 Applications????

Hi All,

I am new to the 454 space and am trying to understand what the best applications for the 454 technology are today with competing read lengths are beginning to creep closer to at least the...
Forum: General 11-30-2012, 06:42 AM
Replies: 0
Views: 1,146
Posted By NGSsequencer
Validation of Illumina on other NGS platforms

Hi we are currently doing our Illumina variant validations using sanger sequencing. Has anyone used another platform like 454 or PGM to validate their results?
It would be human samples that were...
Forum: Illumina/Solexa 09-14-2012, 07:11 AM
Replies: 83
Views: 23,320
Posted By NGSsequencer
Does anyone have any public data sets available...

Does anyone have any public data sets available from their upgraded Miseq?
I'd love to see the quality metrics.
Forum: Core Facilities 09-14-2012, 07:03 AM
Replies: 4
Views: 2,717
Posted By NGSsequencer
Hi Adaptive, Have you found spare capacity?

Hi Adaptive,

Have you found spare capacity?
Forum: Core Facilities 09-14-2012, 07:02 AM
Replies: 9
Views: 6,565
Posted By NGSsequencer
I am also interested in hearing about...

I am also interested in hearing about Otogenetics.
How long does it take to deliver the data back? How was the overall quality and maping?

Are their any alignment options?
Forum: Service Providers 09-14-2012, 06:57 AM
Replies: 9
Views: 5,811
Posted By NGSsequencer
Hi Sklien, Send me a PM, I can help with this

Hi Sklien,

Send me a PM, I can help with this
Forum: Introductions 09-14-2012, 06:41 AM
Replies: 0
Views: 453
Posted By NGSsequencer
Hello

Relatively new. Been a lurker for awhile.
I work for a service provider
interested in exome sequencing, looking to see what people have found using different technologies.

Hello!
Forum: Core Facilities 05-31-2012, 06:21 AM
Replies: 11
Views: 8,787
Posted By NGSsequencer
EdgeBio was recently all over GenomeWeb for CLIA...

EdgeBio was recently all over GenomeWeb for CLIA NGS. Also GeneDx does offers it but that may be too clinical
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