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Forum: Metagenomics 04-16-2018, 11:32 AM
Replies: 1
Views: 317
Posted By LMM
Sanger Sequencing for Community Analysis

Hello
I have environmental cDNA left over from a tricky qPCR project. Is it possible to use Sanger Sequencing of the ITS 5.8S rDNA region to observe the community at even the order level?
Forum: Bioinformatics 01-16-2018, 04:29 PM
Replies: 2
Views: 732
Posted By LMM
Hi Matt Thanks for your input - I'll look into...

Hi Matt
Thanks for your input - I'll look into it.
Cheers
Laura
Forum: Bioinformatics 01-12-2018, 05:22 AM
Replies: 2
Views: 732
Posted By LMM
Laptops for bioinformatics

Hello,

I am a graduate student who is in the market for a new laptop, and I was wondering if anyone had any input. I am currently running (very slowly) Qiime1 & 2 on a smaller dataset from 454,...
Forum: 454 Pyrosequencing 01-11-2018, 06:30 AM
Replies: 2
Views: 1,277
Posted By LMM
Ok thank you!

Ok thank you!
Forum: 454 Pyrosequencing 01-10-2018, 03:03 PM
Replies: 2
Views: 1,277
Posted By LMM
454 data analysis in Qiime1

Hello
I analysing 454 data using Qiime1. I have 4 .fna files that seem to be split into 2 forward read and 2 reverse read files. At what point in the pipeline should I combine them? Before...
Forum: The Pipeline 02-16-2017, 10:50 AM
Replies: 1
Views: 1,731
Posted By LMM
TopHat/ Cufflinks & ...EdgeR?

Hello
Can't seem to find a definite answer to this: Is EdgeR compatible with Cufflinks?
(Im new to this & help is appreciated)
Cheers
Laura
Forum: Illumina/Solexa 01-11-2017, 06:47 AM
Replies: 2
Views: 950
Posted By LMM
Sample size for RNASeq

We are a lab new to sequencing and are preparing for an RNAseq on a soil transcriptome (multiple members, specifically targeting a couple gene families). Given cost constraints, we would like to be...
Forum: Illumina/Solexa 01-10-2017, 06:44 AM
Replies: 1
Views: 831
Posted By LMM
Best/ cheapest Platform for RNA-Seq

I am a MS student working with soil microbiome. I am looking for the presence of a gene family in soils under certain conditions and would like to use RNASeq to profile its expression in soil...
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