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Forum: Ion Torrent 12-18-2015, 02:34 AM
Replies: 7
Views: 3,999
Posted By Xray1
When I get uniformity near 50%, it is usually...

When I get uniformity near 50%, it is usually just that one of two primer pools didn't work. You can check whether amplicons with low coverage are all from the same pool. If so, your ampliseq library...
Forum: Ion Torrent 12-17-2015, 09:08 AM
Replies: 7
Views: 3,999
Posted By Xray1
What panel? As far as I can tell, Ampliseq...

What panel? As far as I can tell, Ampliseq research panels (those designed by Ion or community panels) are usually 125-275bp amplicon range. These should work on Proton.

Since Pipeline Version 4,...
Forum: Ion Torrent 12-17-2015, 03:53 AM
Replies: 7
Views: 3,999
Posted By Xray1
I have no Proton experience myself, but max read...

I have no Proton experience myself, but max read length of Proton is 200bp, isn't it?
What type of application is this, Ampliseq or something else?
Forum: Ion Torrent 11-11-2015, 08:05 AM
Replies: 28
Views: 67,761
Posted By Xray1
As far as I can tell, costs are almost the same...

As far as I can tell, costs are almost the same when comparing PGM-318 vs S5-520 and Proton PI vs S5-540. Correct?

So really what is missing with the S5 is the low-output low-cost option of the...
Forum: Ion Torrent 06-08-2015, 06:09 AM
Replies: 5
Views: 5,282
Posted By Xray1
Hi there, I would like to ask basically the...

Hi there,

I would like to ask basically the same question as Lindsay.

For a larger NGS project with quite a lot of samples but relatively few amplicons (AmpliSeq), I find the idea of the...
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